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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- GTX105741 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105741, RRID:AB_1951013
- Product name
- NPAS2 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 (GTX27900) 7.5% SDS PAGE GTX105741 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NPAS2(GTX105741) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NPAS2 antibody [C1C3] detects NPAS2 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NPAS2(GTX105741) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NPAS2 antibody [C1C3] detects NPAS2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: NPAS2 stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
