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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00003836-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003836-M01, RRID:AB_425518
- Product name
- KPNA1 monoclonal antibody (M01), clone 2A4-1B5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant KPNA1.
- Antigen sequence
MTTPGKENFRLKSYKNKSLNPDEMRRRREEEGLQL
RKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEA
QINNMEMAPGGVITSDMIEMIFSKSPEQQLSATQK
FRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKEN
CTLQFESAWVLTNIASGNSLQTRIVIQAGAVPIFI
ELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDC
NILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSP
PPEFAKVSPCLNVLSWLLFVSDTDVLADACWALSY
LSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSP
ALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSP
KESIKKEACWTISNITAGNRAQIQTVIDANIFPAL
ISILQTAEFRTRKEAAWAITNATSGGSAEQIKYLV
ELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGE
QEAKRNGTGINPYCALIEEAYGLDKIEFLQSHENQ
EIYQKAFDLIEHYFGTEDEDSSIAPQVDLNQQQYI
FQQCEAPMEGFQL- Isotype
- IgG
- Antibody clone number
- 2A4-1B5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box.
Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery.
Nuclear import impairment causes cytoplasmic trans-activation response DNA-binding protein accumulation and is associated with frontotemporal lobar degeneration.
Moriyama T, Sangel P, Yamaguchi H, Obuse C, Miyamoto Y, Oka M, Yoneda Y
Biochemical and biophysical research communications 2015 Jul 3;462(3):201-7
Biochemical and biophysical research communications 2015 Jul 3;462(3):201-7
Targeted disruption of one of the importin α family members leads to female functional incompetence in delivery.
Moriyama T, Nagai M, Oka M, Ikawa M, Okabe M, Yoneda Y
The FEBS journal 2011 May;278(9):1561-72
The FEBS journal 2011 May;278(9):1561-72
Nuclear import impairment causes cytoplasmic trans-activation response DNA-binding protein accumulation and is associated with frontotemporal lobar degeneration.
Nishimura AL, Zupunski V, Troakes C, Kathe C, Fratta P, Howell M, Gallo JM, Hortobágyi T, Shaw CE, Rogelj B
Brain : a journal of neurology 2010 Jun;133(Pt 6):1763-71
Brain : a journal of neurology 2010 Jun;133(Pt 6):1763-71
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KPNA1 monoclonal antibody (M01), clone 2A4-1B5 Western Blot analysis of KPNA1 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of KPNA1 expression in transfected 293T cell line by KPNA1 monoclonal antibody (M01), clone 2A4-1B5.Lane 1: KPNA1 transfected lysate(60.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged KPNA1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to KPNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
