Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Flow cytometry [4]
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Validation data
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- Product number
- NBP2-52414 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Annexin V Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Annexin V Antibody (3B9B7) [NBP2-52414] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - Analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Annexin V Antibody (3B9B7) [NBP2-52414] - An intracellular stain was performed on HeLa cells with Annexin V Antibody (3B9B7) NBP2-52414AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.