Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000308-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000308-M01, RRID:AB_536808
- Product name
- Mouse Monoclonal Annexin V Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ANXA5 - annexin A5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Proteomics and bioinformatics analysis of lovastatin-induced differentiation in ARO cells.
Transcellular distribution heterogeneity of Annexin A5 represents a protective response to lupus-related thrombophilia: a pilot Proteomics-based study.
The differential expression of aqueous soluble proteins in breast normal and cancerous tissues in relation to stage and grade of patients.
Shui HA, Hsia CW, Chen HM, Chang TC, Wang CY
Journal of proteomics 2012 Feb 2;75(4):1170-80
Journal of proteomics 2012 Feb 2;75(4):1170-80
Transcellular distribution heterogeneity of Annexin A5 represents a protective response to lupus-related thrombophilia: a pilot Proteomics-based study.
Zhou D, Luo N, Wu Q, You Y, Zhai Z, Mou Z, Wu Y, Hao F
Biochemical and biophysical research communications 2012 Apr 6;420(2):357-63
Biochemical and biophysical research communications 2012 Apr 6;420(2):357-63
The differential expression of aqueous soluble proteins in breast normal and cancerous tissues in relation to stage and grade of patients.
Liang S, Singh M, Gam LH
Journal of biomedicine & biotechnology 2010;2010:516469
Journal of biomedicine & biotechnology 2010;2010:516469
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody (M01), clone 1F4-1A5.Lane 1: ANXA5 transfected lysate(35.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin V Antibody (1F4-1A5) [H00000308-M01] - ANXA5 monoclonal antibody (M01), clone 1F4-1A5. Analysis of ANXA5 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Detection limit for recombinant GST tagged ANXA5 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Analysis of monoclonal antibody to ANXA5 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Annexin V Antibody (1F4-1A5) [H00000308-M01] - Analysis of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.