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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-57231 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Annexin V Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LVVLLQANRD PDAGIDEAQV EQDAQALFQA GELKWGTDEE KFITIFGTRS VSHLRKVFDK YMTISGFQIE ETIDRETSGN LEQLLLAVVK SIRSIPAYLA ETLYYAMKGA G Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Annexin V in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG; Lane 4: Human plasma; Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an Annexin V Polyclonal Antibody (Product # PA5-57231).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Annexin V was achieved by transfecting HeLa cells with Annexin V specific siRNAs (Silencer® select Product # s1392). Western blot analysis (Fig. a) was performed using whole cell extracts from the Annexin V knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Annexin V Polyclonal Antibody (Product # PA5-57231, 0.4 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Annexin V.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Annexin V Polyclonal Antibody (Product # PA5-57231) and a 35 kDa band corresponding to TFEB was observed along with uncharacterized band (*) in tissue tested. Whole cell extracts of (30 µg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), MDA-MB-231 (Lane 3), Hep G2 (Lane 4) and Mouse Liver (Lane 5) were electrophoresed using Novex®), NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Annexin V in human cell line PC-3 shows positivity in nuclear membrane. Samples were probed using an Annexin V Polyclonal Antibody (Product # PA5-57231).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Annexin V in human placenta and skeletal muscle tissues using Annexin V Polyclonal Antibody (Product # PA5-57231). Corresponding ANXA5 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Annexin V in human prostate using Annexin V Polyclonal Antibody (Product # PA5-57231) shows moderate membranous positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Annexin V in human fallopian tube using Annexin V Polyclonal Antibody (Product # PA5-57231) shows moderate positivity in cilia in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Annexin V in human skeletal muscle using Annexin V Polyclonal Antibody (Product # PA5-57231) shows low positivity in myocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Annexin V in human placenta using Annexin V Polyclonal Antibody (Product # PA5-57231) shows moderate membranous positivity in trophoblastic cells.
