- PA5-27872 - Invitrogen Antibodies ANXA5 antibody

Submitted references Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.

Bhowmick S, Malat A, Caruso D, Ponery N, D'Mello V, Finn C, Abdul-Muneer PM
eNeuro 2021 Jul-Aug;8(4)

Deficiency of mouse mast cell protease 4 mitigates cardiac dysfunctions in mice after myocardium infarction.

Wang Y, Liu CL, Fang W, Zhang X, Yang C, Li J, Liu J, Sukhova GK, Gurish MF, Libby P, Shi GP, Zhang J
Biochimica et biophysica acta. Molecular basis of disease 2019 Jun 1;1865(6):1170-1181

Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish.

Paul CD, Bishop K, Devine A, Paine EL, Staunton JR, Thomas SM, Thomas JR, Doyle AD, Miller Jenkins LM, Morgan NY, Sood R, Tanner K
Cell systems 2019 Aug 28;9(2):187-206.e16

Neurodegeneration and Sensorimotor Deficits in the Mouse Model of Traumatic Brain Injury.

Bhowmick S, D'Mello V, Ponery N, Abdul-Muneer PM
Brain sciences 2018 Jan 6;8(1)

Shielding Engineered Islets With Mesenchymal Stem Cells Enhance Survival Under Hypoxia.

Chandravanshi B, Bhonde RR
Journal of cellular biochemistry 2017 Sep;118(9):2672-2683

Stiffened Extracellular Matrix and Signaling from Stromal Fibroblasts via Osteoprotegerin Regulate Tumor Cell Invasion in a 3-D Tumor in Situ Model.

McLane JS, Ligon LA
Cancer microenvironment : official journal of the International Cancer Microenvironment Society 2016 Dec;9(2-3):127-139

Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment.

McLane JS, Ligon LA
Biophysical journal 2015 Jul 21;109(2):249-64

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Annexin V using A) 30 µg PC-12 whole cell lysate and B) 30 µg Rat2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Annexin V polyclonal antibody (Product # PA5-27872) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Annexin V using 30 µg of A) HeLa S3 and B) HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Annexin V polyclonal antibody (Product # PA5-27872) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Annexin V using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Annexin V polyclonal antibody (Product # PA5-27872) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Annexin V was performed by separating 30 µg of various whole cell lysates by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin V Polyclonal Antibody (Product # PA5-27872) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. A431, B. HeLa , C. HepG2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Annexin V was performed by separating 30 µg of various whole cell lysates by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin V Polyclonal Antibody (Product # PA5-27872) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. GL261, B. C8D30 , C. NIH-3T3 , D. Raw 264.7.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Annexin V was performed by separating 30 µg of whole cell lysates by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin V Polyclonal Antibody (Product # PA5-27872) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. PC-12, B. Rat2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Annexin V was achieved by transfecting HeLa with Annexin V specific siRNAs (Silencer® select Product # s1392). Western blot analysis (Fig. a) was performed using whole cell extracts from the Annexin V knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Annexin V Polyclonal Antibody (Product # PA5-27872, 1:1000 dilution) and chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) . Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Annexin V.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti- Annexin V Polyclonal Antibody (Product # PA5-27872) and a 35kDa band corresponding to Annexin V was observed across the cell lines tested. Whole cell extracts (30ug) of HeLa (Lane 1), MCF-7 (Lane 2), MDA-MB231 (Lane 3), Hep G2 (Lane 4), tissues extracts of Mouse Liver( Lane 5), Mouse Skeletal Muscle (Lane 6) and Rat Skeletal Muscle (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow Cytometry analysis of Annexin V was performed in HeLa cells using Annexin V Polyclonal Antibody (Product # PA5-27872) (red) at a dilution of 1:50. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6. ICAM-1 activation induces cell death in animal and cell models of TBI. A , B , Western blotting of Annexin V and beta-actin in tissue lysates of PFC ( A ), hippocampus ( B ) of WT and ICAM-1 -/- mice 48 h after 10 and 20 psi FPI. C , Western blotting of Annexin V and beta-actin at different time points in the PFC 48 h after injury. D , Western blotting of Annexin V and beta-actin in the cell lysates of hBMVEC 24 h after 3.0 psi stretch injury. Bar graph represents the densitometric ratio of cl-caspase-3 bands versus beta-actin bands ( n = 6/group). E , G , Representative TUNEL staining (green) images 48 h after 10 and 20 psi FPI in the PFC ( E ) and hippocampus ( G ). Scale bar: 25 mum ( E ), 100 mum ( G , bigger panels a-f ), and 20 mum ( G , enlarged panels a1-f1 ); n = 6/group. F , H , Percentage of apoptotic-positive cells in the PFC ( F ) and hippocampus ( H ). I , Trypan blue staining represented as a percentage of dead cell analyzed 24 h after 3.0 psi stretch injury in hBMVEC ( n = 4/group). All values are expressed as mean +- SD one-way ANOVA for C , D , one-way and two-way ANOVA for A , B , F , H followed by Bonferroni post hoc tests. Statistically significant * p < 0.05, ** p < 0.01, *** p < 0.001 versus WT uninjured group in B-D , F-I and versus uninjured hBMVEC in E , J ; # p < 0.05, ## p < 0.01, ### p < 0.001 versus corresponding WT injury groups in B-D , F-I and versus injured hBMVEC in E , J ; @ p < 0.05, @@ p < 0.01, @@@ p < 0.001 versus uninjured IC

PA5-27872

Antibody data