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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF2688 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal ESAM Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human ESAM in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse ESAM is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.
Roesli C, Mumprecht V, Neri D, Detmar M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jun;22(6):1933-44
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jun;22(6):1933-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human ESAM by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ESAM at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of ESAM in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
