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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- PA5-47368 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ESAM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse ESAM is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-human ESAM Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47368) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for ESAM at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ESAM in HUVEC human umbilical vein endothelial cells. Samples were incubated in ESAM polyclonal antibody (Product # PA5-47368) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for ESAM at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ESAM in HUVEC human umbilical vein endothelial cells. Samples were incubated in ESAM polyclonal antibody (Product # PA5-47368) or control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
