H00010797-M01

antibody from Abnova Corporation

Targeting: MTHFD2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010797-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010797-M01, RRID:AB_425867

Product name

MTHFD2 monoclonal antibody (M01), clone 4G7-2G3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MTHFD2.

Antigen sequence

SLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQE
VEEWVASGNKRPHLSVILVGENPASHSYVLNKTRA
AAVVGINSETIMKPASISEEELLNLINKLNNDDNV
DGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVIN
VGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNV
VVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTIS
HRYTPKEQLKKHTILADIVISAAGIPNLITADMIK
EGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQK
AGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEE
REVLKSKELGVATN


Isotype

IgG

Antibody clone number

4G7-2G3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MTHFD2 monoclonal antibody (M01), clone 4G7-2G3 Western Blot analysis of MTHFD2 expression in HepG2 ( Cat # L019V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody (M01), clone 4G7-2G3.Lane 1: MTHFD2 transfected lysate(26.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MTHFD2 is 1 ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol