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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-33200 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33200, RRID:AB_2235593
- Product name
- Rabbit Polyclonal MTHFD2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references KRAS mutation status is associated with enhanced dependency on folate metabolism pathways in non-small cell lung cancer cells.
Moran DM, Trusk PB, Pry K, Paz K, Sidransky D, Bacus SS
Molecular cancer therapeutics 2014 Jun;13(6):1611-24
Molecular cancer therapeutics 2014 Jun;13(6):1611-24
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTHFD2 Antibody [NBP1-33200] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela E: Hep G2 F: Molt-4 G: Raji 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTHFD2 Antibody [NBP1-33200] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MTHFD2 Antibody [NBP1-33200] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MTHFD2 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MTHFD2 Antibody [NBP1-33200] - Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 ug of MTHFD2 antibody [N1C3] Western blot analysis was performed using MTHFD2 antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded human breast carcinoma diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded human MCF7 xenograft. MTHFD2 antibody [N1C3] diluted at 1:500.
