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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PA5-35234 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DMRTA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine based on sequence homology.
- Reactivity
- Human, Mouse, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DMRTA2 in zebra fish brain tissue lysates. Samples were incubated with DMRTA2 polyclonal antibody (Product # PA5-35234). Lysates: 35 µg/lane. DMRTA2 protein (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DMRTA2 showing staining in the microtubules of U-251 cells using a DMRTA2 polyclonal antibody (Product # PA5-35234) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 µg/mL, 10 min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of DMRTA2 in U251 cells. Samples were incubated with DMRTA2 polyclonal antibody (Product # PA5-35234) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.
