PA5-60237

antibody from Invitrogen Antibodies

Targeting: DMRTA2

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-60237

Provider

Invitrogen Antibodies

Product name

DMRTA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GSEAKLQKFD LFPKTLLQAG RPGSPLPPPV KPLSPDGADS GPGTSSPEVR PGSGSENGDG ESF Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 94%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.05 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A novel and sensitive DNA methylation marker for the urine-based liquid biopsies to detect bladder cancer.

Deng L, Chao H, Deng H, Yu Z, Zhao R, Huang L, Gong Y, Zhu Y, Wang Q, Li F, Liu L, He L, Tang Z, Liao C, Qi Y, Wang X, Zeng T, Zou H
BMC cancer 2022 May 6;22(1):510

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DMRTA2 in human colon tissue shows moderate cytoplasmic positivity in glandular cells. Samples were probed using a DMRTA2 Polyclonal Antibody (Product # PA5-60237).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 A DMRTA2 methylation in SV-HUC-1 and various BC cell lines detected by MSP. MSP products in lanes U and M indicate the presence of unmethylated and methylated DMRTA2 , respectively. B Quantification of m DMRTA2 in SV-HUC-1 and various BC cell lines by qMSP with ACTB as the reference gene. Data is shown as mean +- s.d. of three independent experiments ( n = 3). C Level of DMRTA2 mRNA expression in SV-HUC-1 and various BC cell lines by RT-qPCR with GAPDH as the reference gene. Data is shown as mean +- s.d. of three independent experiments ( n = 3). (D) Elevation of DMRTA2 mRNA expression in SV-HUC-1 and various BC cell lines after 5'-Aza-dC treatment (demethylation) by RT-qPCR with GAPDH as the reference gene. Data is shown as mean +- s.d. of three independent experiments ( n = 3). E and F , Level of DMRTA2 protein expression detected in SV-HUC-1 and various BC cell lines by western blot analysis with GAPDH as the internal control. Data is shown as mean +- s.d. of three independent experiments ( n = 3). G and H , Effect of 5'-Aza-dC treatment (demethylation) on DMRTA2 expression in the same set of cell lines by western blot analysis. Data shown as mean +- s.d. of three independent experiments ( n = 3). I and J , Examination of DMRTA2 expression in BC and adjacent normal tissues by IHC. Left panel and right panel show negative and positive staining for two distinct tissue sections. K and L , Morphological features of tumor and adjacent normal tissue sections revealed by H