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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-77776 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATG9B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A 1:1,000 dilution of PA5-77776 was sufficient for detection of ATG9B in 15 µg of Human HeLa Cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 100 kDa.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of ATG9B in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ATG9B polyclonal antibody (Product # PA5-77776) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) ATG9B Antibody D) Merged image. Magnification: 60x.
