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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
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- Product number
- AF1555 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal p27/Kip1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human p27/Kip1 when phosphorylated at T157 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references CACYBP Enhances Cytoplasmic Retention of P27(Kip1) to Promote Hepatocellular Carcinoma Progression in the Absence of RNF41 Mediated Degradation.
Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1.
TRIP6 regulates p27 KIP1 to promote tumorigenesis.
XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt.
Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
Akt isoform-specific inhibition of MDA-MB-231 cell proliferation.
Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
Homeodomain-interacting protein kinase-2 stabilizes p27(kip1) by its phosphorylation at serine 10 and contributes to cell motility.
Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery.
Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor.
Lian YF, Huang YL, Zhang YJ, Chen DM, Wang JL, Wei H, Bi YH, Jiang ZW, Li P, Chen MS, Huang YH
Theranostics 2019;9(26):8392-8408
Theranostics 2019;9(26):8392-8408
Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
Zhao D, Besser AH, Wander SA, Sun J, Zhou W, Wang B, Ince T, Durante MA, Guo W, Mills G, Theodorescu D, Slingerland J
Oncogene 2015 Oct;34(43):5447-59
Oncogene 2015 Oct;34(43):5447-59
SGK3 is an androgen-inducible kinase promoting prostate cancer cell proliferation through activation of p70 S6 kinase and up-regulation of cyclin D1.
Wang Y, Zhou D, Chen S
Molecular endocrinology (Baltimore, Md.) 2014 Jun;28(6):935-48
Molecular endocrinology (Baltimore, Md.) 2014 Jun;28(6):935-48
TRIP6 regulates p27 KIP1 to promote tumorigenesis.
Lin VT, Lin VY, Lai YJ, Chen CS, Liu K, Lin WC, Lin FT
Molecular and cellular biology 2013 Apr;33(7):1394-409
Molecular and cellular biology 2013 Apr;33(7):1394-409
XB130 mediates cancer cell proliferation and survival through multiple signaling events downstream of Akt.
Shiozaki A, Shen-Tu G, Bai X, Iitaka D, De Falco V, Santoro M, Keshavjee S, Liu M
PloS one 2012;7(8):e43646
PloS one 2012;7(8):e43646
Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
Wen S, So Y, Singh K, Slingerland JM, Resnick MB, Zhang S, Ruiz V, Moss SF
Oncogene 2012 Apr 5;31(14):1771-80
Oncogene 2012 Apr 5;31(14):1771-80
Akt isoform-specific inhibition of MDA-MB-231 cell proliferation.
Yang W, Ju JH, Lee KM, Shin I
Cellular signalling 2011 Jan;23(1):19-26
Cellular signalling 2011 Jan;23(1):19-26
Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
Santi SA, Lee H
PloS one 2011 Jan 31;6(1):e14614
PloS one 2011 Jan 31;6(1):e14614
Homeodomain-interacting protein kinase-2 stabilizes p27(kip1) by its phosphorylation at serine 10 and contributes to cell motility.
Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A
The Journal of biological chemistry 2011 Aug 19;286(33):29005-13
The Journal of biological chemistry 2011 Aug 19;286(33):29005-13
Knockdown of protein tyrosine phosphatase SHP-1 inhibits G1/S progression in prostate cancer cells through the regulation of components of the cell-cycle machinery.
Rodríguez-Ubreva FJ, Cariaga-Martinez AE, Cortés MA, Romero-De Pablos M, Ropero S, López-Ruiz P, Colás B
Oncogene 2010 Jan 21;29(3):345-55
Oncogene 2010 Jan 21;29(3):345-55
Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):81-90
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jan 1;15(1):81-90
Overcoming trastuzumab resistance in HER2-overexpressing breast cancer cells by using a novel celecoxib-derived phosphoinositide-dependent kinase-1 inhibitor.
Tseng PH, Wang YC, Weng SC, Weng JR, Chen CS, Brueggemeier RW, Shapiro CL, Chen CY, Dunn SE, Pollak M, Chen CS
Molecular pharmacology 2006 Nov;70(5):1534-41
Molecular pharmacology 2006 Nov;70(5):1534-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Phospho-p27/Kip1 (T157) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 20 minutes. PVDF membrane was probed with 0.5 µg/mL Human Phospho-p27/Kip1 (T157) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-p27/Kip1 (T157) was detected at approximately 27 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase ( lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
