37-9700

antibody from Invitrogen Antibodies

Targeting: CDKN1B KIP1, P27KIP1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

37-9700

Provider

Invitrogen Antibodies

Product name

Phospho-p27 Kip1 (Thr187) Monoclonal Antibody (2B10B7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2B10B7

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

Statin-induced depletion of geranylgeranyl pyrophosphate inhibits cell proliferation by a novel pathway of Skp2 degradation.

Vosper J, Masuccio A, Kullmann M, Ploner C, Geley S, Hengst L
Oncotarget 2015 Feb 20;6(5):2889-902

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PCTAIRE1 phosphorylates p27 and regulates mitosis in cancer cells.

Yanagi T, Krajewska M, Matsuzawa S, Reed JC
Cancer research 2014 Oct 15;74(20):5795-807

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Rb/Cdk2/Cdk4 triple mutant mice elicit an alternative mechanism for regulation of the G1/S transition.

Li W, Kotoshiba S, Berthet C, Hilton MB, Kaldis P
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 13;106(2):486-91

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Targeting the p27 E3 ligase SCF(Skp2) results in p27- and Skp2-mediated cell-cycle arrest and activation of autophagy.

Chen Q, Xie W, Kuhn DJ, Voorhees PM, Lopez-Girona A, Mendy D, Corral LG, Krenitsky VP, Xu W, Moutouh-de Parseval L, Webb DR, Mercurio F, Nakayama KI, Nakayama K, Orlowski RZ
Blood 2008 May 1;111(9):4690-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Raji (Lane 1) and Raji treated with Sodium Vanadate (1mM Sodium vanadate for 30 minutes) (Lane 2). The blot was probed with Anti-Phospho-p27 Kip1 (Thr187) Mouse Monoclonal Antibody (Product # 37-9700, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 27 kDa band corresponding to Phospho-p27 Kip1 (Thr187) was observed in the cell line upon treatment. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).