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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Product number
- 71-7700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-p27 Kip1 (Thr187) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody reacts with the ~ 22 kDa Claudin-1 protein. Reactivity was confirmed by western blotting and immunofluorescence. Positive controls include MDCK cells (dog), Caco-2cells (human), mouse liver, rat liver, and rat kidney. NOTE: This antibody strongly cross reacts with the Claudin-3 protein. It has been observed in western blotting inhibition studies using Caco-2 cell lysates, that this antibody is inhibited by addition of Claudin-3 peptide. For a Claudin-1 specific antibody that does not cross-react with Claudin-3, please order Cat. No. 519000 (Rb x Claudin-1) instead.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/ml
- Storage
- -20°C
Submitted references MARCKS Signaling Differentially Regulates Vascular Smooth Muscle and Endothelial Cell Proliferation through a KIS-, p27kip1- Dependent Mechanism.
Exposure of neuroblastoma cell lines to imatinib results in the upregulation of the CDK inhibitor p27(KIP1) as a consequence of c-Abl inhibition.
High p27 protein levels in chronic lymphocytic leukemia are associated to low Myc and Skp2 expression, confer resistance to apoptosis and antagonize Myc effects on cell cycle.
Human corneal endothelial cells employ phosphorylation of p27(Kip1) at both Ser10 and Thr187 sites for FGF-2-mediated cell proliferation via PI 3-kinase.
SKP2 oncogene is a direct MYC target gene and MYC down-regulates p27(KIP1) through SKP2 in human leukemia cells.
Jun activation domain-binding protein 1 negatively regulate p27 kip1 in non-Hodgkin's lymphomas.
Induction of growth arrest and apoptosis in human breast cancer cells by 3,3-diindolylmethane is associated with induction and nuclear localization of p27kip.
Substrate recognition and ubiquitination of SCFSkp2/Cks1 ubiquitin-protein isopeptide ligase.
Shp-1 mediates the antiproliferative activity of tissue inhibitor of metalloproteinase-2 in human microvascular endothelial cells.
Retinoic acid stabilizes p27Kip1 in EBV-immortalized lymphoblastoid B cell lines through enhanced proteasome-dependent degradation of the p45Skp2 and Cks1 proteins.
A novel route for connexin 43 to inhibit cell proliferation: negative regulation of S-phase kinase-associated protein (Skp 2).
Yu D, Makkar G, Dong T, Strickland DK, Sarkar R, Monahan TS
PloS one 2015;10(11):e0141397
PloS one 2015;10(11):e0141397
Exposure of neuroblastoma cell lines to imatinib results in the upregulation of the CDK inhibitor p27(KIP1) as a consequence of c-Abl inhibition.
Lupino E, Ramondetti C, Buccinnà B, Piccinini M
Biochemical pharmacology 2014 Nov 15;92(2):235-50
Biochemical pharmacology 2014 Nov 15;92(2):235-50
High p27 protein levels in chronic lymphocytic leukemia are associated to low Myc and Skp2 expression, confer resistance to apoptosis and antagonize Myc effects on cell cycle.
Caraballo JM, Acosta JC, Cortés MA, Albajar M, Gómez-Casares MT, Batlle-López A, Cuadrado MA, Onaindia A, Bretones G, Llorca J, Piris MA, Colomer D, León J
Oncotarget 2014 Jul 15;5(13):4694-708
Oncotarget 2014 Jul 15;5(13):4694-708
Human corneal endothelial cells employ phosphorylation of p27(Kip1) at both Ser10 and Thr187 sites for FGF-2-mediated cell proliferation via PI 3-kinase.
Lee JG, Song JS, Smith RE, Kay EP
Investigative ophthalmology & visual science 2011 Oct 17;52(11):8216-23
Investigative ophthalmology & visual science 2011 Oct 17;52(11):8216-23
SKP2 oncogene is a direct MYC target gene and MYC down-regulates p27(KIP1) through SKP2 in human leukemia cells.
Bretones G, Acosta JC, Caraballo JM, Ferrándiz N, Gómez-Casares MT, Albajar M, Blanco R, Ruiz P, Hung WC, Albero MP, Perez-Roger I, León J
The Journal of biological chemistry 2011 Mar 18;286(11):9815-25
The Journal of biological chemistry 2011 Mar 18;286(11):9815-25
Jun activation domain-binding protein 1 negatively regulate p27 kip1 in non-Hodgkin's lymphomas.
Wang Y, Fei M, Cheng C, Zhang D, Lu J, He S, Zhao Y, Wang Y, Shen A
Cancer biology & therapy 2008 Mar;7(3):460-7
Cancer biology & therapy 2008 Mar;7(3):460-7
Induction of growth arrest and apoptosis in human breast cancer cells by 3,3-diindolylmethane is associated with induction and nuclear localization of p27kip.
Wang Z, Yu BW, Rahman KM, Ahmad F, Sarkar FH
Molecular cancer therapeutics 2008 Feb;7(2):341-9
Molecular cancer therapeutics 2008 Feb;7(2):341-9
Substrate recognition and ubiquitination of SCFSkp2/Cks1 ubiquitin-protein isopeptide ligase.
Xu S, Abbasian M, Patel P, Jensen-Pergakes K, Lombardo CR, Cathers BE, Xie W, Mercurio F, Pagano M, Giegel D, Cox S
The Journal of biological chemistry 2007 May 25;282(21):15462-70
The Journal of biological chemistry 2007 May 25;282(21):15462-70
Shp-1 mediates the antiproliferative activity of tissue inhibitor of metalloproteinase-2 in human microvascular endothelial cells.
Seo DW, Li H, Qu CK, Oh J, Kim YS, Diaz T, Wei B, Han JW, Stetler-Stevenson WG
The Journal of biological chemistry 2006 Feb 10;281(6):3711-21
The Journal of biological chemistry 2006 Feb 10;281(6):3711-21
Retinoic acid stabilizes p27Kip1 in EBV-immortalized lymphoblastoid B cell lines through enhanced proteasome-dependent degradation of the p45Skp2 and Cks1 proteins.
Zancai P, Dal Col J, Piccinin S, Guidoboni M, Cariati R, Rizzo S, Boiocchi M, Maestro R, Dolcetti R
Oncogene 2005 Apr 7;24(15):2483-94
Oncogene 2005 Apr 7;24(15):2483-94
A novel route for connexin 43 to inhibit cell proliferation: negative regulation of S-phase kinase-associated protein (Skp 2).
Zhang YW, Nakayama K, Nakayama K, Morita I
Cancer research 2003 Apr 1;63(7):1623-30
Cancer research 2003 Apr 1;63(7):1623-30
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