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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-2816 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2816, RRID:AB_10002085
- Product name
- Goat Polyclonal p27/Kip1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Novel benzylidene-thiazolidine-2,4-diones inhibit Pim protein kinase activity and induce cell cycle arrest in leukemia and prostate cancer cells.
The MLL fusion gene, MLL-AF4, regulates cyclin-dependent kinase inhibitor CDKN1B (p27kip1) expression.
p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion.
Beharry Z, Zemskova M, Mahajan S, Zhang F, Ma J, Xia Z, Lilly M, Smith CD, Kraft AS
Molecular cancer therapeutics 2009 Jun;8(6):1473-83
Molecular cancer therapeutics 2009 Jun;8(6):1473-83
The MLL fusion gene, MLL-AF4, regulates cyclin-dependent kinase inhibitor CDKN1B (p27kip1) expression.
Xia ZB, Popovic R, Chen J, Theisler C, Stuart T, Santillan DA, Erfurth F, Diaz MO, Zeleznik-Le NJ
Proceedings of the National Academy of Sciences of the United States of America 2005 Sep 27;102(39):14028-33
Proceedings of the National Academy of Sciences of the United States of America 2005 Sep 27;102(39):14028-33
p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion.
Baldassarre G, Belletti B, Nicoloso MS, Schiappacassi M, Vecchione A, Spessotto P, Morrione A, Canzonieri V, Colombatti A
Cancer cell 2005 Jan;7(1):51-63
Cancer cell 2005 Jan;7(1):51-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p27/Kip1 Antibody [NB100-2816] - Human Prostate lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p27/Kip1 Antibody [NB100-2816] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
