V2086

antibody from NSJ Bioreagents

Targeting: CDKN1B KIP1, P27KIP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2086

Provider

NSJ Bioreagents

Product name

p27Kip1 Antibody

Antibody type

Monoclonal

Description

This highly specific p27Kip1 antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry/Western blot applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

SX53G8

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse NIH 3T3 cell lysate with p27Kip1 antibody. Expected molecular weight ~27 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse RAW264.7 cell lysate with p27Kip1 antibody. Expected molecular weight ~27 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone SX53G8, green) and Phalloidin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of human colon stained with p27Kip1 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE prostate carcinoma with p27Kip1 antibody

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human prostate carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon carcinoma tissue with p287Kip1 antibody (clone SX53G8). HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS: Intracellular flow testing of HeLa cells with Alexa Fluor 488 conjugated p27Kip1 antibody (green) and isotype control (gray).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Intracellular FACS testing of human HeLa cells with Alexa Fluor 488 conjugated p27Kip1 antibody (clone SX53G8, green) and isotype control (gray).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry staining of PFA-fixed human MCF7 cells with p27Kip1 antibody; Red=isotype control, Blue= p27Kip1 antibody.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone SX53G8) as confirmation of integrity and purity.