Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-32889-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal p27/Kip1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p27 KIP 1 Antibody (SX53G8) [NBP2-32889] - Formalin-paraffin human colon stained with p27 MAb (SX53G8)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Formain-fixed, paraffin-embedded human prostate Carcinoma stained with p27 Monoclonal Antibody (sx53G8)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Flow cytometry of human p27 on HeLa cells.Black:cells alone; Grey; isotype control;Green; AF488-labeled p27 Monoclonal Antibody (SX53G8)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: p27/Kip1 Antibody (SX53G8) [NBP2-32889] - Analysis of PFA-fixed MCF-7 cells using p27/Kip1 Antibody (SX53G8) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).