M00173-1

antibody from Boster Biological Technology

Targeting: CDKN1B KIP1, P27KIP1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00173-1

Provider

Boster Biological Technology

Product name

Anti-p27 KIP 1 CDKN1B Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for p27/CDKN1B detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Rat p27/CDKN1B information: Molecular Weight: 22073 MW; Subcellular Localization: Nucleus. Cytoplasm. Endosome . Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity); Tissue Specificity: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Reactivity

Human, Rat

Host

Rabbit

Antibody clone number

CGA-3

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Metallothionein 1 family profiling identifies MT1X as a tumor suppressor involved in the progression and metastastatic capacity of hepatocellular carcinoma.

Liu Z, Ye Q, Wu L, Gao F, Xie H, Zhou L, Zheng S, Xu X
Molecular carcinogenesis 2018 Nov;57(11):1435-1444

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17-beta-hydroxysteroid dehydrogenase 13 inhibits the progression and recurrence of hepatocellular carcinoma.

Chen J, Zhuo JY, Yang F, Liu ZK, Zhou L, Xie HY, Xu X, Zheng SS
Hepatobiliary & pancreatic diseases international : HBPD INT 2018 Jun;17(3):220-226

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RASSF1A promotes apoptosis and suppresses the proliferation of ovarian cancer cells.

Fu L, Zhang S
International journal of molecular medicine 2014 May;33(5):1153-60

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using p27 KIP 1 Antibody(M00173-1)CDKN1B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDKN1B Antibody (M00173-1)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image