Antibody data
- Antibody Data
- Antigen structure
- References [48]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Flow cytometry [3]
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Validation data
Reference
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- Product number
- MA5-12835 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-p27Kip1 Monoclonal Antibody (DCS-72.F6)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-12835 targets p27Kip1 in FACS, IF, IP, and WB applications and shows reactivity with Canine, Human, mouse, and Rat samples. The MA5-12835 immunogen is mouse recombinant p27 protein.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- DCS-72.F6
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references The RNA-binding protein LIN28B regulates developmental timing in the mammalian cochlea.
Apigenin inhibits prostate cancer progression in TRAMP mice via targeting PI3K/Akt/FoxO pathway.
Recruitment of trimeric proliferating cell nuclear antigen by G1-phase cyclin-dependent kinases following DNA damage with platinum-based antitumour agents.
MiR-223 suppresses cell proliferation by targeting IGF-1R.
Inflammatory mediators of esophagitis alter p27 Kip1 expression in esophageal epithelial cells.
Presence of HER4 associates with increased sensitivity to Herceptin in patients with metastatic breast cancer.
Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis.
Penta-O-galloyl-beta-D-glucose induces S- and G(1)-cell cycle arrests in prostate cancer cells targeting DNA replication and cyclin D1.
Upregulation of TNF receptor type 2 in human and experimental renal allograft rejection.
Progressive changes in hepatoma cells stably transfected with hepatitis B virus X gene.
The combination of high cyclin E and Skp2 expression in breast cancer is associated with a poor prognosis and the basal phenotype.
Activated Akt as an indicator of prognosis in gastric cancer.
Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry.
BAF180 is a critical regulator of p21 induction and a tumor suppressor mutated in breast cancer.
Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.
Expression of senescence-related genes in human corneal endothelial cells.
Disruption of heparan and chondroitin sulfate signaling enhances mesenchymal stem cell-derived osteogenic differentiation via bone morphogenetic protein signaling pathways.
Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells.
Antitumor activity of CTFB, a novel anticancer agent, is associated with the down-regulation of nuclear factor-kappaB expression and proteasome activation in head and neck squamous carcinoma cell lines.
Increased SKP2 and CKS1 gene expression contributes to the progression of human urothelial carcinoma.
Prognostic implications of cyclin B1, p34cdc2, p27(Kip1) and p53 expression in gastric cancer.
Increased expression of CCAAT/enhancer-binding protein beta in proliferative inflammatory atrophy of the prostate: relation with the expression of COX-2, the androgen receptor, and presence of focal chronic inflammation.
Prospective identification and purification of hair cell and supporting cell progenitors from the embryonic cochlea.
E Proteins and Id2 converge on p57Kip2 to regulate cell cycle in neural cells.
Iressa induces cytostasis and augments Fas-mediated apoptosis in acinic cell adenocarcinoma overexpressing HER2/neu.
N-all-trans-retinoyl-L-proline inhibits metastatic potential of hepatocellular carcinoma cells.
Suppressor of cytokine signalling 2 (SOCS-2) expression in breast carcinoma.
Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells.
An in vivo evaluation of docetaxel delivered intratumorally in head and neck squamous cell carcinoma.
Expression of p8 protein in breast carcinoma; an inverse relationship with apoptosis.
Significantly decreased P27 expression in endometrial carcinoma compared to complex hyperplasia with atypia (correlation with p53 expression).
Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.
Expression of Islet1 marks the sensory and neuronal lineages in the mammalian inner ear.
Silibinin protects against photocarcinogenesis via modulation of cell cycle regulators, mitogen-activated protein kinases, and Akt signaling.
pRb2/p130, vascular endothelial growth factor, p27(KIP1), and proliferating cell nuclear antigen expression in hepatocellular carcinoma: their clinical significance.
Predicting early failure after adjuvant chemotherapy in high-risk breast cancer patients with extensive lymph node involvement.
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
In vitro growth and differentiation of mammalian sensory hair cell progenitors: a requirement for EGF and periotic mesenchyme.
Simultaneous chromosome 1q gain and 16q loss is associated with steroid receptor presence and low proliferation in breast carcinoma.
A pharmacodynamic study of the epidermal growth factor receptor tyrosine kinase inhibitor ZD1839 in metastatic colorectal cancer patients.
Improved endometrial assessment using cyclin E and p27.
The role of Math1 in inner ear development: Uncoupling the establishment of the sensory primordium from hair cell fate determination.
Expression of cell-cycle-regulated proteins pRb2/p130, p107, p27(kip1), p53, mdm-2, and Ki-67 (MIB-1) in prostatic gland adenocarcinoma.
p27Kip1 localizes to detergent-insoluble microdomains within lymphocyte membranes.
Insulin-like growth factor-I receptor signaling and resistance to trastuzumab (Herceptin).
p27Kip1 expression is associated with clinical outcome in advanced epithelial ovarian cancer: multivariate analysis.
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2.
Golden EJ, Benito-Gonzalez A, Doetzlhofer A
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 21;112(29):E3864-73
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 21;112(29):E3864-73
Apigenin inhibits prostate cancer progression in TRAMP mice via targeting PI3K/Akt/FoxO pathway.
Shukla S, Bhaskaran N, Babcook MA, Fu P, Maclennan GT, Gupta S
Carcinogenesis 2014 Feb;35(2):452-60
Carcinogenesis 2014 Feb;35(2):452-60
Recruitment of trimeric proliferating cell nuclear antigen by G1-phase cyclin-dependent kinases following DNA damage with platinum-based antitumour agents.
He G, Kuang J, Koomen J, Kobayashi R, Khokhar AR, Siddik ZH
British journal of cancer 2013 Oct 29;109(9):2378-88
British journal of cancer 2013 Oct 29;109(9):2378-88
MiR-223 suppresses cell proliferation by targeting IGF-1R.
Jia CY, Li HH, Zhu XC, Dong YW, Fu D, Zhao QL, Wu W, Wu XZ
PloS one 2011;6(11):e27008
PloS one 2011;6(11):e27008
Inflammatory mediators of esophagitis alter p27 Kip1 expression in esophageal epithelial cells.
Nguyen KD, Blain SW, Gress F, Treem WR
Journal of pediatric gastroenterology and nutrition 2010 Nov;51(5):556-62
Journal of pediatric gastroenterology and nutrition 2010 Nov;51(5):556-62
Presence of HER4 associates with increased sensitivity to Herceptin in patients with metastatic breast cancer.
Sassen A, Diermeier-Daucher S, Sieben M, Ortmann O, Hofstaedter F, Schwarz S, Brockhoff G
Breast cancer research : BCR 2009;11(4):R50
Breast cancer research : BCR 2009;11(4):R50
Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis.
Zhang W, Zeng Z, Zhou Y, Xiong W, Fan S, Xiao L, Huang D, Li Z, Li D, Wu M, Li X, Shen S, Wang R, Cao L, Tang K, Li G
Acta biochimica et biophysica Sinica 2009 May;41(5):414-28
Acta biochimica et biophysica Sinica 2009 May;41(5):414-28
Penta-O-galloyl-beta-D-glucose induces S- and G(1)-cell cycle arrests in prostate cancer cells targeting DNA replication and cyclin D1.
Hu H, Zhang J, Lee HJ, Kim SH, Lü J
Carcinogenesis 2009 May;30(5):818-23
Carcinogenesis 2009 May;30(5):818-23
Upregulation of TNF receptor type 2 in human and experimental renal allograft rejection.
Hoffmann U, Bergler T, Rihm M, Pace C, Krüger B, Rümmele P, Stoelcker B, Banas B, Männel DN, Krämer BK
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2009 Apr;9(4):675-86
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2009 Apr;9(4):675-86
Progressive changes in hepatoma cells stably transfected with hepatitis B virus X gene.
Ye L, Dong N, Wang Q, Xu Z, Cai N, Wang H, Zhang X
Intervirology 2008;51(1):50-8
Intervirology 2008;51(1):50-8
The combination of high cyclin E and Skp2 expression in breast cancer is associated with a poor prognosis and the basal phenotype.
Voduc D, Nielsen TO, Cheang MC, Foulkes WD
Human pathology 2008 Oct;39(10):1431-7
Human pathology 2008 Oct;39(10):1431-7
Activated Akt as an indicator of prognosis in gastric cancer.
Cinti C, Vindigni C, Zamparelli A, La Sala D, Epistolato MC, Marrelli D, Cevenini G, Tosi P
Virchows Archiv : an international journal of pathology 2008 Nov;453(5):449-55
Virchows Archiv : an international journal of pathology 2008 Nov;453(5):449-55
Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry.
Shi SR, Liu C, Pootrakul L, Tang L, Young A, Chen R, Cote RJ, Taylor CR
American journal of clinical pathology 2008 Mar;129(3):358-66
American journal of clinical pathology 2008 Mar;129(3):358-66
BAF180 is a critical regulator of p21 induction and a tumor suppressor mutated in breast cancer.
Xia W, Nagase S, Montia AG, Kalachikov SM, Keniry M, Su T, Memeo L, Hibshoosh H, Parsons R
Cancer research 2008 Mar 15;68(6):1667-74
Cancer research 2008 Mar 15;68(6):1667-74
Differential modification of p27Kip1 controls its cyclin D-cdk4 inhibitory activity.
James MK, Ray A, Leznova D, Blain SW
Molecular and cellular biology 2008 Jan;28(1):498-510
Molecular and cellular biology 2008 Jan;28(1):498-510
Expression of senescence-related genes in human corneal endothelial cells.
Song Z, Wang Y, Xie L, Zang X, Yin H
Molecular vision 2008 Jan 29;14:161-70
Molecular vision 2008 Jan 29;14:161-70
Disruption of heparan and chondroitin sulfate signaling enhances mesenchymal stem cell-derived osteogenic differentiation via bone morphogenetic protein signaling pathways.
Manton KJ, Leong DF, Cool SM, Nurcombe V
Stem cells (Dayton, Ohio) 2007 Nov;25(11):2845-54
Stem cells (Dayton, Ohio) 2007 Nov;25(11):2845-54
Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells.
Wiseman DA, Werner SR, Crowell PL
The Journal of pharmacology and experimental therapeutics 2007 Mar;320(3):1163-70
The Journal of pharmacology and experimental therapeutics 2007 Mar;320(3):1163-70
Antitumor activity of CTFB, a novel anticancer agent, is associated with the down-regulation of nuclear factor-kappaB expression and proteasome activation in head and neck squamous carcinoma cell lines.
Skvortsov S, Skvortsova I, Stasyk T, Schiefermeier N, Neher A, Gunkel AR, Bonn GK, Huber LA, Lukas P, Pleiman CM, Zwierzina H
Molecular cancer therapeutics 2007 Jun;6(6):1898-908
Molecular cancer therapeutics 2007 Jun;6(6):1898-908
Increased SKP2 and CKS1 gene expression contributes to the progression of human urothelial carcinoma.
Kawakami K, Enokida H, Tachiwada T, Nishiyama K, Seki N, Nakagawa M
The Journal of urology 2007 Jul;178(1):301-7
The Journal of urology 2007 Jul;178(1):301-7
Prognostic implications of cyclin B1, p34cdc2, p27(Kip1) and p53 expression in gastric cancer.
Kim DH
Yonsei medical journal 2007 Aug 31;48(4):694-700
Yonsei medical journal 2007 Aug 31;48(4):694-700
Increased expression of CCAAT/enhancer-binding protein beta in proliferative inflammatory atrophy of the prostate: relation with the expression of COX-2, the androgen receptor, and presence of focal chronic inflammation.
Wang W, Bergh A, Damber JE
The Prostate 2007 Aug 1;67(11):1238-46
The Prostate 2007 Aug 1;67(11):1238-46
Prospective identification and purification of hair cell and supporting cell progenitors from the embryonic cochlea.
Doetzlhofer A, White P, Lee YS, Groves A, Segil N
Brain research 2006 May 26;1091(1):282-8
Brain research 2006 May 26;1091(1):282-8
E Proteins and Id2 converge on p57Kip2 to regulate cell cycle in neural cells.
Rothschild G, Zhao X, Iavarone A, Lasorella A
Molecular and cellular biology 2006 Jun;26(11):4351-61
Molecular and cellular biology 2006 Jun;26(11):4351-61
Iressa induces cytostasis and augments Fas-mediated apoptosis in acinic cell adenocarcinoma overexpressing HER2/neu.
Piechocki MP, Yoo GH, Dibbley SK, Amjad EH, Lonardo F
International journal of cancer 2006 Jul 15;119(2):441-54
International journal of cancer 2006 Jul 15;119(2):441-54
N-all-trans-retinoyl-L-proline inhibits metastatic potential of hepatocellular carcinoma cells.
Wu XZ, Shi PC, Hu P, Chen Y, Ding SS
Cell biology international 2006 Aug;30(8):672-80
Cell biology international 2006 Aug;30(8):672-80
Suppressor of cytokine signalling 2 (SOCS-2) expression in breast carcinoma.
Farabegoli F, Ceccarelli C, Santini D, Taffurelli M
Journal of clinical pathology 2005 Oct;58(10):1046-50
Journal of clinical pathology 2005 Oct;58(10):1046-50
Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells.
Siitonen T, Koistinen P, Savolainen ER
Leukemia research 2005 Nov;29(11):1335-42
Leukemia research 2005 Nov;29(11):1335-42
An in vivo evaluation of docetaxel delivered intratumorally in head and neck squamous cell carcinoma.
Yoo GH, Subramanian G, Boinpally RR, Iskander A, Shehadeh N, Oliver J, Ezzat W, Piechocki MP, Ensley JF, Lin HS, Shibuya TY, Polin L, Parchment RE
Archives of otolaryngology--head & neck surgery 2005 May;131(5):418-29
Archives of otolaryngology--head & neck surgery 2005 May;131(5):418-29
Expression of p8 protein in breast carcinoma; an inverse relationship with apoptosis.
Ito Y, Yoshida H, Motoo Y, Iovanna JL, Nakamura Y, Kakudo K, Uruno T, Takamura Y, Miya A, Noguchi S, Kuma K, Miyauchi A
Anticancer research 2005 Mar-Apr;25(2A):833-7
Anticancer research 2005 Mar-Apr;25(2A):833-7
Significantly decreased P27 expression in endometrial carcinoma compared to complex hyperplasia with atypia (correlation with p53 expression).
Ozkara SK, Corakci A
Pathology oncology research : POR 2004;10(2):89-97
Pathology oncology research : POR 2004;10(2):89-97
Evaluation of immunohistochemical markers in non-small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers.
Au NH, Cheang M, Huntsman DG, Yorida E, Coldman A, Elliott WM, Bebb G, Flint J, English J, Gilks CB, Grimes HL
The Journal of pathology 2004 Sep;204(1):101-9
The Journal of pathology 2004 Sep;204(1):101-9
Expression of Islet1 marks the sensory and neuronal lineages in the mammalian inner ear.
Radde-Gallwitz K, Pan L, Gan L, Lin X, Segil N, Chen P
The Journal of comparative neurology 2004 Sep 27;477(4):412-21
The Journal of comparative neurology 2004 Sep 27;477(4):412-21
Silibinin protects against photocarcinogenesis via modulation of cell cycle regulators, mitogen-activated protein kinases, and Akt signaling.
Mallikarjuna G, Dhanalakshmi S, Singh RP, Agarwal C, Agarwal R
Cancer research 2004 Sep 1;64(17):6349-56
Cancer research 2004 Sep 1;64(17):6349-56
pRb2/p130, vascular endothelial growth factor, p27(KIP1), and proliferating cell nuclear antigen expression in hepatocellular carcinoma: their clinical significance.
Claudio PP, Russo G, Kumar CA, Minimo C, Farina A, Tutton S, Nuzzo G, Giuliante F, Angeloni G, Maria V, Vecchio FM, Campli CD, Giordano A
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 May 15;10(10):3509-17
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 May 15;10(10):3509-17
Predicting early failure after adjuvant chemotherapy in high-risk breast cancer patients with extensive lymph node involvement.
Faneyte IF, Peterse JL, Van Tinteren H, Pronk C, Bontenbal M, Beex LV, van der Wall E, Richel DJ, Nooij MA, Voest EE, Hupperets P, Ten Vergert EM, de Vries EG, Rodenhuis S, van de Vijver MJ
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Jul 1;10(13):4457-63
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Jul 1;10(13):4457-63
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
Qiu LQ, Sinniah R, I-Hong Hsu S
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):79-90
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):79-90
In vitro growth and differentiation of mammalian sensory hair cell progenitors: a requirement for EGF and periotic mesenchyme.
Doetzlhofer A, White PM, Johnson JE, Segil N, Groves AK
Developmental biology 2004 Aug 15;272(2):432-47
Developmental biology 2004 Aug 15;272(2):432-47
Simultaneous chromosome 1q gain and 16q loss is associated with steroid receptor presence and low proliferation in breast carcinoma.
Farabegoli F, Hermsen MA, Ceccarelli C, Santini D, Weiss MM, Meijer GA, van Diest PJ
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2004 Apr;17(4):449-55
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2004 Apr;17(4):449-55
A pharmacodynamic study of the epidermal growth factor receptor tyrosine kinase inhibitor ZD1839 in metastatic colorectal cancer patients.
Daneshmand M, Parolin DA, Hirte HW, Major P, Goss G, Stewart D, Batist G, Miller WH Jr, Matthews S, Seymour L, Lorimer IA
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Jul;9(7):2457-64
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Jul;9(7):2457-64
Improved endometrial assessment using cyclin E and p27.
Dubowy RL, Feinberg RF, Keefe DL, Doncel GF, Williams SC, McSweet JC, Kliman HJ
Fertility and sterility 2003 Jul;80(1):146-56
Fertility and sterility 2003 Jul;80(1):146-56
The role of Math1 in inner ear development: Uncoupling the establishment of the sensory primordium from hair cell fate determination.
Chen P, Johnson JE, Zoghbi HY, Segil N
Development (Cambridge, England) 2002 May;129(10):2495-505
Development (Cambridge, England) 2002 May;129(10):2495-505
Expression of cell-cycle-regulated proteins pRb2/p130, p107, p27(kip1), p53, mdm-2, and Ki-67 (MIB-1) in prostatic gland adenocarcinoma.
Claudio PP, Zamparelli A, Garcia FU, Claudio L, Ammirati G, Farina A, Bovicelli A, Russo G, Giordano GG, McGinnis DE, Giordano A, Cardi G
Clinical cancer research : an official journal of the American Association for Cancer Research 2002 Jun;8(6):1808-15
Clinical cancer research : an official journal of the American Association for Cancer Research 2002 Jun;8(6):1808-15
p27Kip1 localizes to detergent-insoluble microdomains within lymphocyte membranes.
Yaroslavskiy BB, Stolz DB, Watkins SC, Alber SM, Bradbury NA, Steinman RA
Molecular medicine (Cambridge, Mass.) 2001 Jan;7(1):49-58
Molecular medicine (Cambridge, Mass.) 2001 Jan;7(1):49-58
Insulin-like growth factor-I receptor signaling and resistance to trastuzumab (Herceptin).
Lu Y, Zi X, Zhao Y, Mascarenhas D, Pollak M
Journal of the National Cancer Institute 2001 Dec 19;93(24):1852-7
Journal of the National Cancer Institute 2001 Dec 19;93(24):1852-7
p27Kip1 expression is associated with clinical outcome in advanced epithelial ovarian cancer: multivariate analysis.
Masciullo V, Ferrandina G, Pucci B, Fanfani F, Lovergine S, Palazzo J, Zannoni G, Mancuso S, Scambia G, Giordano A
Clinical cancer research : an official journal of the American Association for Cancer Research 2000 Dec;6(12):4816-22
Clinical cancer research : an official journal of the American Association for Cancer Research 2000 Dec;6(12):4816-22
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
Tsutsui T, Hesabi B, Moons DS, Pandolfi PP, Hansel KS, Koff A, Kiyokawa H
Molecular and cellular biology 1999 Oct;19(10):7011-9
Molecular and cellular biology 1999 Oct;19(10):7011-9
The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2.
Reynisdóttir I, Massagué J
Genes & development 1997 Feb 15;11(4):492-503
Genes & development 1997 Feb 15;11(4):492-503
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of p27Kip1 using p27Kip1 Monoclonal Antibody (MA5-12835) on LS174T Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of p27Kip1 using p27Kip1 Monoclonal Antibody (MA5-12835) on Native Mouse MAD109 Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of p27Kip1 in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # MA5-12835) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of p27Kip1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # MA5-12835) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of p27Kip1 in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p27Kip1 monoclonal antibody (Product # MA5-12835) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.