Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-13254 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p27 Kip1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with hamster based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.39 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Molecular Consequences of Depression Treatment: A Potential In Vitro Mechanism for Antidepressants-Induced Reprotoxic Side Effects.
Neuronal life or death linked to depression treatment: the interplay between drugs and their stress-related outcomes relate to single or combined drug therapies.
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes.
Sołek P, Mytych J, Tabęcka-Łonczyńska A, Koziorowski M
International journal of molecular sciences 2021 Nov 1;22(21)
International journal of molecular sciences 2021 Nov 1;22(21)
Neuronal life or death linked to depression treatment: the interplay between drugs and their stress-related outcomes relate to single or combined drug therapies.
Solek P, Koszla O, Mytych J, Badura J, Chelminiak Z, Cuprys M, Fraczek J, Tabecka-Lonczynska A, Koziorowski M
Apoptosis : an international journal on programmed cell death 2019 Oct;24(9-10):773-784
Apoptosis : an international journal on programmed cell death 2019 Oct;24(9-10):773-784
Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.
Mytych J, Wos I, Solek P, Koziorowski M
Experimental cell research 2017 Jan 15;350(2):358-367
Experimental cell research 2017 Jan 15;350(2):358-367
Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes.
Mytych J, Romerowicz-Misielak M, Koziorowski M
Toxicology in vitro : an international journal published in association with BIBRA 2017 Aug;42:1-9
Toxicology in vitro : an international journal published in association with BIBRA 2017 Aug;42:1-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a 27Kip1 polyclonal antibody (Product # PA5-13254) in HepG2 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a 27Kip1 polyclonal antibody (Product # PA5-13254) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Antidepressants-mediated effect on cellular protein content. HT-22 cells were treated with antidepressants for 48 and 96 h and densitometry analysis of NF-kappaB ( b ), p16 ( c ), p21 ( d ), p27 ( e ), p53 ( f ), TRF1 ( g ), TRF2 ( h ), calnexin ( i ), NuMa ( j ), cleaved caspase 3 ( k ), Bcl-2 ( l ) was evaluated. Representative Western Blots are presented ( a ). Bars indicate SD, n = 3, *** /^^^ p < 0.001, ** /^^ p < 0.01, * /^ p < 0.05, no indication--no statistical significance (one-way ANOVA and Dunnett's a posteriori test)