PA5-27188

antibody from Invitrogen Antibodies

Targeting: CDKN1B KIP1, P27KIP1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27188

Provider

Invitrogen Antibodies

Product name

p27 Kip1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HeLa, MCF-7, MDA-MB-231, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12. Predicted reactivity: Mouse (80%), Dog (87%), Cat (87%), Pig (84%), Rhesus Monkey (98%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.07 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Human 3D cellular model of hypoxic brain injury of prematurity.

Pașca AM, Park JY, Shin HW, Qi Q, Revah O, Krasnoff R, O'Hara R, Willsey AJ, Palmer TD, Pașca SP
Nature medicine 2019 May;25(5):784-791

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CDKN1B/p27 is localized in mitochondria and improves respiration-dependent processes in the cardiovascular system-New mode of action for caffeine.

Ale-Agha N, Goy C, Jakobs P, Spyridopoulos I, Gonnissen S, Dyballa-Rukes N, Aufenvenne K, von Ameln F, Zurek M, Spannbrucker T, Eckermann O, Jakob S, Gorressen S, Abrams M, Grandoch M, Fischer JW, Köhrer K, Deenen R, Unfried K, Altschmied J, Haendeler J
PLoS biology 2018 Jun;16(6):e2004408

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ESRP1 Mutations Cause Hearing Loss due to Defects in Alternative Splicing that Disrupt Cochlear Development.

Rohacek AM, Bebee TW, Tilton RK, Radens CM, McDermott-Roe C, Peart N, Kaur M, Zaykaner M, Cieply B, Musunuru K, Barash Y, Germiller JA, Krantz ID, Carstens RP, Epstein DJ
Developmental cell 2017 Nov 6;43(3):318-331.e5

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Context-Dependent Functional Divergence of the Notch Ligands DLL1 and DLL4 In Vivo.

Preuße K, Tveriakhina L, Schuster-Gossler K, Gaspar C, Rosa AI, Henrique D, Gossler A, Stauber M
PLoS genetics 2015 Jun;11(6):e1005328

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of p27 Kip1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 Polyclonal Antibody (Product # PA5 27188) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of p27 Kip1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: p27 Kip1 Polyclonal Antibody (Product # PA5 27188) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ulcerative colitis tissue using anti-p27 Kip1 Polyclonal Antibody (Product # PA5-27188).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Frozen) analysis of p27-Kip1 was performed in frozen sectioned adult mouse retina tissue using p27 Kip1 Polyclonal Antibody (Product # PA5-27188) at a dilution of 1:250 (Green). Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 4 DLL4 expressed from the Dll1 locus rescues DLL1 loss-of-function in the retina. (A) Dll1 mutant retinas show epithelial disruption with formation of polarised rosettes in which the apical markers N-Cadherin (NCad, a) and ZO-1 (ZO1, b) are abnormally present at the central lumen. Ectopic proliferating progenitors, labelled with PHH3 (b, arrowheads), are located close to the apical lumen of these rosettes. (B) In contrast, the neuroepithelium of homozygous Dll1 Dll1ki and Dll1 Dll4ki embryos is correctly organised without rosettes, and N-Cadherin shows the normal apical localisation close to the retinal pigmented epithelium (a,b). Mitotic progenitors (PHH3+) are only detected at the apical region of the neuroepithelium (a,b arrowheads). A normal stratification of CHX10+ progenitors and P27+ differentiating neurons is also observed (c,d). (C, D) E13.5 homozygous Dll1 Dll1ki and Dll1 Dll4ki retinas show no significant difference in the number of ISL1+ RGCs (C) and CRABP+ amacrine cells (D). Cells immunopositive for Islet-1 and Crabp were counted and related to the total number of cells in the retina (DAPI+). Percentages are shown as mean +- SEM; ns, not significant. (E) Expression of DLL4 in homozygous Dll1 Dll1ki (a,c) and in homozygous Dll1 Dll4ki (b,d) E13.5 retinas as detected by an anti-DLL4 antibody. (c) and (d) are magnifications of (a) and (b), respectively. Endogenous plus transgenic DLL4 is expressed in more cells in Dll1 Dll4ki/Dll4ki as compared to endogeno