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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-32530 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p27 Kip1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, mouse and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is prostate or colon tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of p27Kip1 using anti-p27Kip1 Polyclonal Antibody (Product # PA5-32530) in Prostate Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
