Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- NB600-1442 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1442, RRID:AB_791444
- Product name
- Rabbit Polyclonal p27/Kip1 Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 80 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Sirolimus affects cardiomyocytes to reduce left ventricular mass in heart transplant recipients.
Kushwaha SS, Raichlin E, Sheinin Y, Kremers WK, Chandrasekaran K, Brunn GJ, Platt JL
European heart journal 2008 Nov;29(22):2742-50
European heart journal 2008 Nov;29(22):2742-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p27/Kip1 Antibody [NB600-1442] - Lane 1: HEK293 pcDNA3. Lane 2: HEK293 pcDNA3 Rb2wt. Lane 3: HEK293 pcDNA3 Rb2S941A. Lane 4: HEK293 pcDNA3 Rb2 PM19. Load: 30 ug per lane. Primary antibody: p27 antibody diluted in 1%BSA at 1:2000 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for p27.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p27/Kip1 Antibody [NB600-1442] - Analysis of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight 800 conjugated Gt-a-Rabbit IgG.