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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-60708 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal EI24 Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4degreesC.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: EI24 Antibody [NBP2-60708] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EI24 antibody NBP2-60708 used for IP at 8 ul per reaction. EI24 was also immunoprecipitated by rabbit anti-EI24 antibody NBP2-60709. For blotting immunoprecipitated EI24, NBP2-60708 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
