12034-1-AP

antibody from Proteintech Group

Targeting: STT3A ITM1, MGC9042, STT3-A, TMC

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12034-1-AP

Provider

Proteintech Group

Product name

STT3A antibody

Antibody type

Polyclonal

Description

KD/KO validated STT3A antibody (Cat. #12034-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Truncated N-glycans destabilize POMT1 and POMT2 but do not limit cellular O-mannosylation in HEK293 cells.

Sturm D, Hölscher C, Martínez IA, Harst A, Konstantinidi A, Alam S, Burock R, Vakhrushev SY, Hoffmann M, Ruppert T, Rapp E, Halim A, Thiel C, Strahl S
Molecular genetics and metabolism 2026 Jun;148(2):110115

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Structural basis of regulated N-glycosylation at the secretory translocon.

Yamsek M, Ma M, Jha R, Wan Y, Li Q, Zhong F, DeLong K, Ji Z, Rohatgi R, Keenan RJ
Nature 2026 Jan;649(8097):777-784

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STT3A-mediated FCN3 N-glycosylation promotes Treg cell activation to drive hepatocellular carcinoma progression via Wnt/β-catenin.

Zhang H, Zhang P, Gong T, Zhang C, Wang Z, Zhao Y
Cellular oncology (Dordrecht, Netherlands) 2026 Jan 6;49(1):16

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Construction of a glycosylation-related prognostic signature for predicting prognosis, tumor microenvironment, and immune response in soft tissue sarcoma.

Yuan N, Zhang J, Yuan J, Xie Z, Gao Y, Raza M, Gao Y, Zuo G
Frontiers in oncology 2025;15:1636830

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Integrated screening identifies GPR31 as a key driver and druggable target for metabolic dysfunction-associated steatohepatitis.

Zhang XJ, Fu J, Cheng X, Shen H, Yang H, Wang K, Li W, Tian H, Tian T, Zhou J, Tian S, Wang Z, Wan J, Bai L, Duan H, Zhang X, Tian R, Xu H, Liao R, Zou T, Shi J, Qu W, Fang L, Cai J, Zhang P, She ZG, Jiang J, Hu Y, Wang Y, Li H
The Journal of clinical investigation 2025 Sep 2;135(17)

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Pharmacological Dissection Identifies Retatrutide Overcomes the Therapeutic Barrier of Obese TNBC Treatments through Suppressing the Interplay between Glycosylation and Ubiquitylation of YAP.

Cui X, Zhu Y, Zeng L, Zhang M, Uddin A, Gillespie TW, McCullough LE, Zhao S, Torres MA, Wan Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Mar;12(11):e2407494

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Redundancy of the OST catalytic subunit facilitates therapeutic targeting of N-glycosylation.

Baro M, Lee H, Kelley V, Lou R, Phoomak C, Politi K, Zeiss CJ, Van Zandt M, Contessa JN
Cell chemical biology 2025 Jun 19;32(6):839-853.e6

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Interferon-inducible guanylate-binding protein 5 inhibits replication of multiple viruses by binding to the oligosaccharyltransferase complex and inhibiting glycoprotein maturation.

Wang S, Li W, Wen J, Wang L, Tiwari SK, Bray W, Wu L, Li N, Hui H, Clark AE, Zhang Q, Zhang L, Carlin AF, Rana TM
mBio 2025 Dec 10;16(12):e0293025

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STT3-mediated aberrant N-glycosylation of CD24 inhibits paclitaxel sensitivity in triple-negative breast cancer.

Wang J, Zhang HM, Zhu GH, Zhao LL, Shi J, Dai ZT, Li JP, Li XR, Sun F, Wu Y, Chen SY, Li HN, Liao XH, Xiang Y
Acta pharmacologica Sinica 2025 Apr;46(4):1097-1110

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Interferon-Inducible Guanylate-Binding Protein 5 Inhibits Replication of Multiple Viruses by Binding to the Oligosaccharyltransferase Complex and Inhibiting Glycoprotein Maturation.

Wang S, Li W, Wang L, Tiwari SK, Bray W, Wu L, Li N, Hui H, Clark AE, Zhang Q, Zhang L, Carlin AF, Rana TM
bioRxiv : the preprint server for biology 2024 May 3;

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MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning.

Wang Q, Su Y, Sun R, Xiong X, Guo K, Wei M, Yang G, Ru Y, Zhang Z, Li J, Zhang J, Qiao Q, Li X
Cell & bioscience 2024 Jan 20;14(1):12

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Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB.

Lampson BL, Ramίrez AS, Baro M, He L, Hegde M, Koduri V, Pfaff JL, Hanna RE, Kowal J, Shirole NH, He Y, Doench JG, Contessa JN, Locher KP, Kaelin WG Jr
Cell 2024 Apr 25;187(9):2209-2223.e16

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Identification of a Novel Prognostic Signature Based on N-Linked Glycosylation and Its Correlation with Immunotherapy Response in Hepatocellular Carcinoma.

Lin S, Cao Y, Zhu K, Yang C, Zhu X, Zhang H, Zhang R
Journal of hepatocellular carcinoma 2023;10:1749-1765

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STT3A-mediated viral N-glycosylation underlies the tumor selectivity of oncolytic virus M1.

Song D, Jia X, Gao Y, Xiao T, Dan J, Shen R, Cai J, Liang J, Zhu W, Hu J, Yan G, Zhang Q, Lin Y
Oncogene 2023 Nov;42(48):3575-3588

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Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation.

Phoomak C, Rinis N, Baro M, Shrimal S, Bennett D, Shaffer SA, Lehrman M, Gilmore R, Contessa JN
Science advances 2023 Mar 17;9(11):eade8079

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Deglycosylation of SLAMF7 in breast cancers enhances phagocytosis.

Wang SH, Chou WC, Huang HC, Lee TA, Hsiao TC, Wang LH, Huang KB, Kuo CT, Chao CH, Chang SJ, Hsu JM, Weng J, Ren N, Li FA, Lai YJ, Zhou C, Hung MC, Li CW
American journal of cancer research 2022;12(10):4721-4736

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TGF-β1-Mediated PD-L1 Glycosylation Contributes to Immune Escape via c-Jun/STT3A Pathway in Nasopharyngeal Carcinoma.

Ma XM, Luo YF, Zeng FF, Su C, Liu X, Li XP, Lu J
Frontiers in oncology 2022;12:815437

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Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.

Wright MT, Kouba L, Plate L
Molecular & cellular proteomics : MCP 2021;20:100008

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Mettl14-Mediated m(6)A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis.

Cao X, Shu Y, Chen Y, Xu Q, Guo G, Wu Z, Shao M, Zhou Y, Chen M, Gong Y, Li C, Shi Y, Bu H
Cellular and molecular gastroenterology and hepatology 2021;12(2):633-651

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Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.

Huang HC, Lai YJ, Liao CC, Yang WF, Huang KB, Lee IJ, Chou WC, Wang SH, Wang LH, Hsu JM, Sun CP, Kuo CT, Wang J, Hsiao TC, Yang PJ, Lee TA, Huang W, Li FA, Shen CY, Lin YL, Tao MH, Li CW
EBioMedicine 2021 Dec;74:103712

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Intramembrane protease RHBDL4 cleaves oligosaccharyltransferase subunits to target them for ER-associated degradation.

Knopf JD, Landscheidt N, Pegg CL, Schulz BL, Kühnle N, Chao CW, Huck S, Lemberg MK
Journal of cell science 2020 Mar 26;133(6)

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Pharmacologic Suppression of B7-H4 Glycosylation Restores Antitumor Immunity in Immune-Cold Breast Cancers.

Song X, Zhou Z, Li H, Xue Y, Lu X, Bahar I, Kepp O, Hung MC, Kroemer G, Wan Y
Cancer discovery 2020 Dec;10(12):1872-1893

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The AT1 receptor autoantibody causes hypoglycemia in fetal rats via promoting the STT3A-GLUT1-glucose uptake axis in liver.

Wang P, He C, Yue M, Wang T, Bai L, Wu Y, Liu D, Wang M, Sun Y, Li Y, Zhang S, Liu H
Molecular and cellular endocrinology 2020 Dec 1;518:111022

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Construction of green fluorescence protein mutant to monitor STT3B-dependent N-glycosylation.

Kitajima T, Xue W, Liu YS, Wang CD, Liu SS, Fujita M, Gao XD
The FEBS journal 2018 Mar;285(5):915-928

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A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.

Sasaoka N, Imamura H, Kakizuka A
iScience 2018 Dec 21;10:211-221

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Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.

Lin DL, Cherepanova NA, Bozzacco L, MacDonald MR, Gilmore R, Tai AW
mBio 2017 Jul 18;8(4)

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Changes in cellular glycosylation of leukemia cells upon treatment with acridone derivatives yield insight into drug action.

Wang Y, Park D, Galermo AG, Gao D, Liu H, Lebrilla CB
Proteomics 2016 Dec;16(23):2977-2988

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Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Lopez-Sambrooks C, Shrimal S, Khodier C, Flaherty DP, Rinis N, Charest JC, Gao N, Zhao P, Wells L, Lewis TA, Lehrman MA, Gilmore R, Golden JE, Contessa JN
Nature chemical biology 2016 Dec;12(12):1023-1030

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12034-1-AP(STT3A antibody) at dilution of 1:1000

Other comments

WB

Appendix

12034-1-AP.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human pancreas cancer using 12034-1-AP(STT3A antibody) at dilution of 1:50 (under 10x lens)

Other comments

IHC

Appendix

12034-1-AP.pdf

Show more