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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-96993 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HYLS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YPHVESNVPS ETVSEASQRL RKPVMKRKVL RRKPDGEVLV TDESIISESE SGTENDQDLW DLRQRLMNVQ FQEDKESSFD VSQKFNLPHE YQGISQDQLI CSLQREGMGS PAYEQDLIVA SRPKSFILPK LDQLSRNRGK TDRVARYFEY KRDWDSIRLP GEDHRKELRW GVREQMLCRA EPQSKPQHIY VPNNYLVPTE KKRSALRWGV RCDLANGVIP RKLPFPLSPS; Positive Samples: MCF-7; Cellular Location: Cytoplasm
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.53 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HYLS1 in extracts of MCF-7 cells. Samples were incubated with HYLS1 Polyclonal antibody (Product # PA5-96993) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HYLS1 was performed in U2OS cells using HYLS1 Polyclonal Antibody (Product # PA5-96993) at a dilution of 1:100. Blue: DAPI for nuclear staining.
