PA5-19305

antibody from Invitrogen Antibodies

Targeting: POU3F3 BRN1, OTF8

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19305

Provider

Invitrogen Antibodies

Product name

Anti-POU3F3/BRN1/OCT8

Antibody type

Polyclonal

Antigen

Synthetic peptide sequence (HMLSHAHQWVTAL) corresponding to the internal amino acids of POU3F3

Reactivity

Rat

Host

Goat

Vial size

100 ug

Concentration

0.5 mg/ml

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of POU3F3 using POU3F3 Polyclonal Antibody (Product # PA5-19305) (1 µg/mL) in staining of Mouse Spinal Cord (A) and Mouse Brain (B) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of POU3F3 using POU3F3 Polyclonal Antibody (Product # PA5-19305) in paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of POU3F3 using POU3F3 Polyclonal Antibody (Product # PA5-19305) in paraformaldehyde fixed Neuro-2a cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.