14-9813-81

antibody from Invitrogen Antibodies

Targeting: GP6 GPVI

Western blot (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

14-9813-81

Provider

Invitrogen Antibodies

Product name

GP6 Monoclonal Antibody (HY101), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The HY101 monoclonal antibody reacts with the glycoprotein VI (GPVI), a 62-kDa type-I transmembrane glycoprotein that is expressed on mature megakaryotes and platelets. GPVI associates with the Fc receptor (FcR) gamma-chain to form a high affinity receptor for collagen. GPVI has also been reported to bind convulxin (Cvx). Phosphorylation of the ITAMs present on the FcR gamma-chain leads to recruitment of Src family kinases and activation of downstream signal transduction. GPVI has been reported to play a critical role in platelet activation and aggregation. Applications Reported: This HY101 antibody has been reported for use in flow cytometric analysis, and western blotting. Applications Tested: This HY101 antibody has been tested by flow cytometric analysis or immunoblotting. In flow cytometry this can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HY101

Vial size

50 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

A compound heterozygous mutation in glycoprotein VI in a patient with a bleeding disorder.

Hermans C, Wittevrongel C, Thys C, Smethurst PA, Van Geet C, Freson K
Journal of thrombosis and haemostasis : JTH 2009 Aug;7(8):1356-63

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Platelet glycoprotein VI: its structure and function.

Moroi M, Jung SM
Thrombosis research 2004;114(4):221-33

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GPVI levels in platelets: relationship to platelet function at high shear.

Best D, Senis YA, Jarvis GE, Eagleton HJ, Roberts DJ, Saito T, Jung SM, Moroi M, Harrison P, Green FR, Watson SP
Blood 2003 Oct 15;102(8):2811-8

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Fc Rgamma -independent signaling by the platelet collagen receptor glycoprotein VI.

Locke D, Liu C, Peng X, Chen H, Kahn ML
The Journal of biological chemistry 2003 Apr 25;278(17):15441-8

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The platelet receptor GPVI mediates both adhesion and signaling responses to collagen in a receptor density-dependent fashion.

Chen H, Locke D, Liu Y, Liu C, Kahn ML
The Journal of biological chemistry 2002 Jan 25;277(4):3011-9