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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- F54770 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NUB1 Antibody / NEDD8 ultimate buster 1 / NYREN18
- Antibody type
- Polyclonal
- Description
- This highly specific NUB1 antibody is suitable for use in Western blot/Immunohistochemistry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the NYREN18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse liver lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with NUB1 antibody. Expected molecular weight: 70-100 kDa depending on level of ubiquitination.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human cancer tissue with NUB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
