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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30516 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NARF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, MCF-7. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NARF Polyclonal Antibody (Product # PA5-30516). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: MCF-7 . 7.5% SDS PAGE. NARF Polyclonal Antibody (Product # PA5-30516) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NARF in paraformaldehyde-fixed HeLa cells using a NARF polyclonal antibody (Product # PA5-30516) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NARF Polyclonal Antibody detects NARF protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NARF Polyclonal Antibody (Product # PA5-30516) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
