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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX111804 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111804, RRID:AB_11175381
- Product name
- GTF2H1 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE GTX111804 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with GTF2H1 antibody [N1C1] (GTX111804) diluted at 1:1000 and competitor's antibody (SC-292) diluted by 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GTF2H1 antibody [N1C1] detects GTF2H1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GTF2H1 stained by GTF2H1 antibody [N1C1] (GTX111804) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10£gm.
