PA1-41167

antibody from Invitrogen Antibodies

Targeting: IL20RA IL-20R1, ZCYTOR7

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41167

Provider

Invitrogen Antibodies

Product name

IL20RA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with chimpanzee and rat samples based on sequence homology. Suggested positive control: A375.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Osteocytes regulate neutrophil development through IL-19: a potent cytokine for neutropenia treatment.

Xiao M, Zhang W, Liu W, Mao L, Yang J, Hu L, Zhang S, Zheng Y, Liu A, Song Q, Li Y, Xiao G, Zou Z, Bai X
Blood 2021 Jun 24;137(25):3533-3547

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis ofIL-20RA in A375 cell lysate in the (A)absence and (B) presence of blocking peptide using a IL-20 Receptor alpha polyclonal antibody (Product # PA1-41167) at2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-375 (Lane 1), MCF7 (Lane 2), SH-SY5Y (Lane 3), HeLa (Lane 4), Hep G2 (Lane 5), PC 12 (Lane 6) and tissue extract of Mouse Colon (Lane 7). The blot was probed with Anti-IL20RA Polyclonal Antibody (Product # PA1-41167, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 80 kDa band corresponding to IL20RA was observed across all the cell lines and tissue tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IL20RA in A375 cell lysate in the (A) absence and (B) presence of blocking peptide. Samples were incubated in IL20RA polyclonal antibody (Product # PA1-41167) using a dilution of 2 µg/mL.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell-surface flow cytometry analysis of IL-20RA in A375 cells using a IL-20 Receptor alpha polyclonal antibody (Product # PA1-41167) at 5 µg/10^6 cells.  The shaded histogram represents cells only, green represents isotype control, and purple represents IL-20 Receptor alpha polyclonal antibody (Product # PA1-41167).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of IL20RA in A375 cells. Samples were incubated in IL20RA polyclonal antibody (Product # PA1-41167) using a dilution of 5 µg/10^6 cells. The shaded histogram represents cells only, green represents isotype control, and purple represents anti-IL-20 R alpha Antibody.