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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [2]
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- Product number
- NB600-863-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Lactate Dehydrogenase Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].
- Reactivity
- Rabbit
- Host
- Goat
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lactate Dehydrogenase Antibody [HRP] [NB600-863] - Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Lactate Dehydrogenase Antibody [HRP] [NB600-863] - Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.
