MAB9205-100

antibody from R&D Systems

Targeting: LDHB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB9205-100

Provider

R&D Systems

Product name

Human Lactate Dehydrogenase B/LDHB Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects human LDHB in direct ELISAs and Western blots.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

P07195


Isotype

IgG

Antibody clone number

2057D

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Lactate Dehydrogenase B/LDHB by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human Lactate Dehydrogenase B/LDHB Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LDHB knockout HEK293T cell line (KO). PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line. Lactate Dehydrogenase B/LDHB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Lactate Dehydrogenase B/LDHB in Human Liver. Lactate Dehydrogenase B/LDHB was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.