A21974

antibody from Invitrogen Antibodies

Targeting: CPT2 CPT1, CPTASE

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A21974

Provider

Invitrogen Antibodies

Product name

CPT2 Monoclonal Antibody (1C2AE6)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat, Bovine

Host

Mouse

Isotype

IgG

Antibody clone number

1C2AE6

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 4 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 4 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.