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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-12217 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CPT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma models.
Loss of macrophage fatty acid oxidation does not potentiate systemic metabolic dysfunction.
Nguyen TTT, Zhang Y, Shang E, Shu C, Torrini C, Zhao J, Bianchetti E, Mela A, Humala N, Mahajan A, Harmanci AO, Lei Z, Maienschein-Cline M, Quinzii CM, Westhoff MA, Karpel-Massler G, Bruce JN, Canoll P, Siegelin MD
The Journal of clinical investigation 2020 Jul 1;130(7):3699-3716
The Journal of clinical investigation 2020 Jul 1;130(7):3699-3716
Loss of macrophage fatty acid oxidation does not potentiate systemic metabolic dysfunction.
Gonzalez-Hurtado E, Lee J, Choi J, Selen Alpergin ES, Collins SL, Horton MR, Wolfgang MJ
American journal of physiology. Endocrinology and metabolism 2017 May 1;312(5):E381-E393
American journal of physiology. Endocrinology and metabolism 2017 May 1;312(5):E381-E393
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using a CPT2 polyclonal antibody (Product # PA5-12217) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Generation of mice with a macrophage-specific loss of mitochondrial fatty acid ß-oxidation. A: mRNA for carnitine palmitoyltransferase (CPT) II (Cpt2) in bone marrow-derived macrophages (BMDM) from control [wild-type (WT)] mice and BMDM from mice with a specific knockout of Cpt2 [CPT2 Mɸ-KO (KO), n = 3]. B: Western blot for Cpt2 in control and CPT2 Mɸ-KO BMDM. C: Western blot of mitochondrial proteins in control and CPT2 Mɸ-KO BMDM in the presence and absence of IL-4 stimulation (n = 2). D: oxidation of [1-14C]oleic acid to 14CO2 in control and CPT2 Mɸ-KO BMDM (n = 6). E: oxidation of [U-14C]glucose to 14CO2 in control and CPT2 Mɸ-KO BMDM (n = 6). F: incorporation of [3H]acetate into lipid in control and CPT2 Mɸ-KO BMDM (n = 6). G: [1,2-3H]2-deoxyglucose uptake in control and CPT2 Mɸ-KO BMDM (n = 6). H: steady-state metabolite concentrations in IL-4- or LPS-IFNγ-stimulated control and CPT2 Mɸ-KO BMDM (n = 5-6). Values are means SE. *P < 0.05, ***P < 0.001 for genotype comparison. #P < 0.05, ###P < 0.001 for treatment comparison.
