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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB49451 - Provider product page
- Provider
- R&D Systems
- Product name
- Human APLP-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human APLP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse APLP-2 or recombinant human APLP-1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q06481- Isotype
- IgG
- Antibody clone number
- 397702
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
DeVay RM, Yamamoto L, Shelton DL, Liang H
PloS one 2015;10(4):e0125127
PloS one 2015;10(4):e0125127
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human APLP-2 by Western Blot. Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Human APLP-2 Monoclonal Antibody (Catalog # MAB49451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APLP-2 at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
