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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [2]
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- Product number
- RQ5892 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ApoER2 Antibody / LRP8
- Antibody type
- Polyclonal
- Description
- This highly specific ApoER2 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ApoER2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) placenta, 2) U-87 MG, 3) A549, 4) U937, 5) HL-60, 6) A431, 7) HeLa, 8) rat testis, 9) rat thymus, 10) rat spleen, 11) rat heart, 12) mouse testis, 13) mouse thymus, 14) mouse heart and 15) mouse RAW264.7 lysate with ApoER2 antibody. Predicted molecular weight ~106 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HepG2 cells with ApoER2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer with ApoER2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with ApoER2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer with ApoER2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse intestine with ApoER2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat intestine with ApoER2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with ApoER2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ApoER2 antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HL-60 cells with ApoER2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ApoER2 antibody.
