Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-34722 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNRPA Recombinant Rabbit Monoclonal Antibody (JB33-48)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Rat brain tissue lysate, K562, A431, mouse kidney tissue lysate, 293T, SH-SY-5Y, SiHa human lung cancer tissue, mouse liver tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB33-48
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of U1A in Lane 1: Rat brain, Lane 2: K562, Lane 3: A431, Lane 4: Mouse kidney. Samples were incubated with U1A monoclonal antibody (Product # MA5-34722), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U1A in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with U1A monoclonal antibody (Product # MA5-34722), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U1A in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with U1A monoclonal antibody (Product # MA5-34722), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U1A in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with U1A monoclonal antibody (Product # MA5-34722), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of U1A in paraffin-embedded mouse liver tissue. Samples were incubated with U1A monoclonal antibody (Product # MA5-34722), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of U1A in paraffin-embedded human lung cancer tissue. Samples were incubated with U1A monoclonal antibody (Product # MA5-34722), and followed by hematoxylin.