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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
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- Product number
- PA5-27474 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNRPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2.
- Concentration
- 1 mg/mL
Submitted references tRIP-seq reveals repression of premature polyadenylation by co-transcriptional FUS-U1 snRNP assembly.
SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome.
Masuda A, Kawachi T, Takeda JI, Ohkawara B, Ito M, Ohno K
EMBO reports 2020 May 6;21(5):e49890
EMBO reports 2020 May 6;21(5):e49890
SRSF1 and hnRNP H antagonistically regulate splicing of COLQ exon 16 in a congenital myasthenic syndrome.
Rahman MA, Azuma Y, Nasrin F, Takeda J, Nazim M, Bin Ahsan K, Masuda A, Engel AG, Ohno K
Scientific reports 2015 Aug 18;5:13208
Scientific reports 2015 Aug 18;5:13208
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SNRPA Polyclonal Antibody (Product # PA5-27474). Sample (30 µg of whole cell lysate). Lane A: A549. Lane B: HeLa. Lane C: HepG2. 12% SDS PAGE. SNRPA Polyclonal Antibody (Product # PA5-27474) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SNRPA was achieved by transfecting A-431 with SNRPA specific siRNAs (Silencer® select Product # s13213, s13214). Western blot analysis (Fig. a) was performed using whole cell extracts from the SNRPA knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SNRPA Polyclonal Antibody (Product # PA5-27474, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SNRPA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell lysates (1% SDS) of A549 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), MOLT-4 (Lane 4), NTERA-2 (Lane 5), K-562 (Lane 6), U-2 OS (Lane 7), SH-SY5Y (Lane 8) and A-431 (Lane 9). The blot was probed with SNRPA Polyclonal Antibody (Product # PA5-27474 , 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 32 kDa band corresponding to SNRPA was observed across the panel tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U1A in paraformaldehyde-fixed HeLa cells using a U1A polyclonal antibody (Product # PA5-27474) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SNRPA was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SNRPA Polyclonal Antibody (Product # PA5-27474) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using SNRPA (Product # PA5-27474) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPA Polyclonal Antibody detects SNRPA protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SNRPA Polyclonal Antibody (Product # PA5-27474) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPA Polyclonal Antibody detects SNRPA protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SNRPA Polyclonal Antibody (Product # PA5-27474) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPA Polyclonal Antibody detects SNRPA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. SNRPA Polyclonal Antibody (Product # PA5-27474) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SNRPA Polyclonal Antibody detects SNRPA protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SNRPA Polyclonal Antibody (Product # PA5-27474) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
