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- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Flow cytometry [1]
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- Product number
- AF3946 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Matriptase/ST14 Catalytic Domain Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Matriptase/ST14 Catalytic Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Matriptase/Epithin is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y5Y6- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Conserved function of the matriptase-prostasin proteolytic cascade during epithelial morphogenesis.
Inflammatory cytokines down-regulate the barrier-protective prostasin-matriptase proteolytic cascade early in experimental colitis.
Matriptase zymogen supports epithelial development, homeostasis and regeneration.
Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated Prostasin.
Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors.
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Regulation of feto-maternal barrier by matriptase- and PAR-2-mediated signaling is required for placental morphogenesis and mouse embryonic survival.
Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epithelium.
Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.
Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation.
c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome.
Loss of matriptase suppression underlies spint1 mutation-associated ichthyosis and postnatal lethality.
Drees L, Königsmann T, Jaspers MHJ, Pflanz R, Riedel D, Schuh R
PLoS genetics 2019 Jan;15(1):e1007882
PLoS genetics 2019 Jan;15(1):e1007882
Inflammatory cytokines down-regulate the barrier-protective prostasin-matriptase proteolytic cascade early in experimental colitis.
Buzza MS, Johnson TA, Conway GD, Martin EW, Mukhopadhyay S, Shea-Donohue T, Antalis TM
The Journal of biological chemistry 2017 Jun 30;292(26):10801-10812
The Journal of biological chemistry 2017 Jun 30;292(26):10801-10812
Matriptase zymogen supports epithelial development, homeostasis and regeneration.
Friis S, Tadeo D, Le-Gall SM, Jürgensen HJ, Sales KU, Camerer E, Bugge TH
BMC biology 2017 Jun 1;15(1):46
BMC biology 2017 Jun 1;15(1):46
Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated Prostasin.
Friis S, Madsen DH, Bugge TH
The Journal of biological chemistry 2016 Feb 5;291(6):2577-82
The Journal of biological chemistry 2016 Feb 5;291(6):2577-82
Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors.
Sales KU, Friis S, Abusleme L, Moutsopoulos NM, Bugge TH
Oncogene 2015 Aug 27;34(35):4664-72
Oncogene 2015 Aug 27;34(35):4664-72
Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling.
Zoratti GL, Tanabe LM, Varela FA, Murray AS, Bergum C, Colombo É, Lang JE, Molinolo AA, Leduc R, Marsault E, Boerner J, List K
Nature communications 2015 Apr 15;6:6776
Nature communications 2015 Apr 15;6:6776
Regulation of feto-maternal barrier by matriptase- and PAR-2-mediated signaling is required for placental morphogenesis and mouse embryonic survival.
Szabo R, Peters DE, Kosa P, Camerer E, Bugge TH
PLoS genetics 2014 Jul;10(7):e1004470
PLoS genetics 2014 Jul;10(7):e1004470
Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epithelium.
Beaulieu A, Gravel É, Cloutier A, Marois I, Colombo É, Désilets A, Verreault C, Leduc R, Marsault É, Richter MV
Journal of virology 2013 Apr;87(8):4237-51
Journal of virology 2013 Apr;87(8):4237-51
Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.
Szabo R, Uzzun Sales K, Kosa P, Shylo NA, Godiksen S, Hansen KK, Friis S, Gutkind JS, Vogel LK, Hummler E, Camerer E, Bugge TH
PLoS genetics 2012;8(8):e1002937
PLoS genetics 2012;8(8):e1002937
Endogenous expression of matriptase in neural progenitor cells promotes cell migration and neuron differentiation.
Fang JD, Chou HC, Tung HH, Huang PY, Lee SL
The Journal of biological chemistry 2011 Feb 18;286(7):5667-79
The Journal of biological chemistry 2011 Feb 18;286(7):5667-79
c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase.
Szabo R, Rasmussen AL, Moyer AB, Kosa P, Schafer JM, Molinolo AA, Gutkind JS, Bugge TH
Oncogene 2011 Apr 28;30(17):2003-16
Oncogene 2011 Apr 28;30(17):2003-16
Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome.
Sales KU, Masedunskas A, Bey AL, Rasmussen AL, Weigert R, List K, Szabo R, Overbeek PA, Bugge TH
Nature genetics 2010 Aug;42(8):676-83
Nature genetics 2010 Aug;42(8):676-83
Loss of matriptase suppression underlies spint1 mutation-associated ichthyosis and postnatal lethality.
Szabo R, Kosa P, List K, Bugge TH
The American journal of pathology 2009 Jun;174(6):2015-22
The American journal of pathology 2009 Jun;174(6):2015-22
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Matriptase/ST14 Catalytic Domain in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Sheep Anti-Human Matriptase/ST14 Catalytic Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3946, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
