Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM09138PU-S - Provider product page
- Provider
- OriGene
- Product name
- UBE2S mouse monoclonal antibody, clone AT2C12, Purified
- Antibody type
- Monoclonal
- Description
- UBE2S mouse monoclonal antibody, clone AT2C12, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- UBE2S
- Isotype
- IgG
- Antibody clone number
- AT2C12
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of 293T (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2S (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cell lysates of 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2S (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green).
- Validation comment
- IF
