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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- A301-796A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RanBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse RanBP2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody (Product # A301-796A; lot A301-796A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RanBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP2 antibody A301-796A (lot A301-796A-2) used for IP at 6 µg per reaction. RanBP2 was also immunoprecipitated by rabbit anti-RanBP2 antibody A301-797A. For blotting immunoprecipitated RanBP2, A301-796A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse RanBP2 by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody A301-796A (lot A301-796A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RanBP2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody A301-796A (lot A301-796A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RanBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP2 antibody (Product # A301-796A; lot A301-796A-3) used for IP at 6 µg per reaction. RanBP2 was also immunoprecipitated by a previous lot of this antibody (lot A301-796A-2) and rabbit anti-RanBP2 antibody (Product # A301-797A). For blotting immunoprecipitated RanBP2, Product # A301-796A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
