PA1-082

antibody from Invitrogen Antibodies

Targeting: RANBP2 ADANE, ANE1, NUP358

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-082

Provider

Invitrogen Antibodies

Product name

RANBP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-082 detects Ran binding protein 2 (RanBP2) from human and bovine retina. PA1-082 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~360 kDa protein representing RanBP2 from bovine retinal extracts. Immunohistochemical staining of bovine retina with PA1-082 results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells. The PA1-082 immunogen is a synthetic peptide corresponding to residues M(1) R R S K A D V E R Y I A S V Q G S(18) of human RanBP2. This sequence is completely conserved between human and mouse. The PA1-082 immunizing peptide (Cat. # PEP-137) is available for use in neutralization and control experiments.

Reactivity

Human, Bovine

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Species-specific vulnerability of RanBP2 shaped the evolution of SIV as it transmitted in African apes.

Meyerson NR, Warren CJ, Vieira DASA, Diaz-Griferro F, Sawyer SL
PLoS pathogens 2018 Mar;14(3):e1006906

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Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis.

Asally M, Yasuda Y, Oka M, Otsuka S, Yoshimura SH, Takeyasu K, Yoneda Y
The FEBS journal 2011 Feb;278(4):610-21

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Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity.

Hamard PJ, Boyer-Guittaut M, Camuzeaux B, Dujardin D, Hauss C, Oelgeschläger T, Vigneron M, Kedinger C, Chatton B
Nucleic acids research 2007;35(4):1134-44

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of RanBP2 in bovine retina using Product # PA1-082.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 7 HIV-1, SIVcpz, and SIVgor are equally dependent on RanBP2. A) 293T cells were infected with lentivirus harboring the indicated shRNA constructs and MTT-proliferation assays were carried out in triplicate at 96 hours post infection. Absorbance was measured at a wavelength of 590 nm. Reduced absorbance is indicative of reduced cell proliferation. B) Whole cell extract (WCE) from 293T cells infected with lentivirus harboring the indicated shRNA construct was collected at 72 hours post infection and immunoblotted with antibodies raised against RanBP2 or Nup153 as a loading control. C) 293T cells treated with the indicated shRNA constructs were infected with HIV-1 bearing the indicated cyclophilin-binding loop (X-axis) and a GFP reporter. The percentage cells infected in each sample was normalized to the shRNA control. Infections were performed in triplicate and error bars represent twice the standard error of the mean.