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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-24252 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAMTS15 Monoclonal Antibody (561819)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 561819
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADAMTS15 in MCF‚7 human breast cancer cell line. Samples were incubated in ADAMTS15 monoclonal antibody (Product # MA5-24252) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for mature ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADAMTS15 in immersion fixed paraffin-embedded sections of human kidney. Samples were incubated in ADAMTS15 monoclonal antibody (Product # MA5-24252) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 human breast cancer cell line was stained with human ADAMTS15 Monoclonal Antibody (Product # MA5-24252, filled histogram) or isotype control antibodyopen histogram), followed by Phycoerythrin-conjugated Anti-mouse IgG F (ab')2 Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ADAMTS15 in MCF‚7 human breast cancer cell line. Samples were incubated in ADAMTS15 monoclonal antibody (Product # MA5-24252) or isotype control antibody followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab)2 Secondary Antibody.
