15732-1-AP

antibody from Proteintech Group

Targeting: HSPB11 C1orf41, FAP232, HSPCO34, IFT25, PP25

Provider product page for 15732-1-AP

Western blot

ELISA

Antibody data

Antibody Data
Antigen structure
References [17]
Comments [0]
Validations
Western blot [1]

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Product number: 15732-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15732-1-AP, RRID:AB_2279933
Product name: IFT25 antibody
Antibody type: Polyclonal
Description: KD/KO validated IFT25 antibody (Cat. #15732-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HSPB11 protein structure - 15732-1-AP shown in red.

Submitted references Dynein-2-driven intraciliary retrograde trafficking indirectly requires multiple interactions of IFT54 in the IFT-B complex with the dynein-2 complex.

Hiyamizu S, Qiu H, Tsurumi Y, Hamada Y, Katoh Y, Nakayama K
Biology open 2023 Jul 15;12(7)

KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1 Deficiency-Related Polycystic Kidney Disease.

Rah G, Cha H, Kim J, Song J, Kim H, Oh YK, Ahn C, Kang M, Kim J, Yoo KH, Kim MJ, Ko HW, Ko JY, Park JH
Journal of the American Society of Nephrology : JASN 2022 Sep;33(9):1726-1741

Rab34 is necessary for early stages of intracellular ciliogenesis.

Stuck MW, Chong WM, Liao JC, Pazour GJ
Current biology : CB 2021 Jul 12;31(13):2887-2894.e4

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Duan S, Li H, Zhang Y, Yang S, Chen Y, Qiu B, Huang C, Wang J, Li J, Zhu X, Yan X
The EMBO journal 2021 Jan 15;40(2):e105499

The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility.

Qu W, Yuan S, Quan C, Huang Q, Zhou Q, Yap Y, Shi L, Zhang D, Guest T, Li W, Yee SP, Zhang L, Cazin C, Hess RA, Ray PF, Kherraf ZE, Zhang Z
American journal of physiology. Cell physiology 2020 Jun 1;318(6):C1092-C1106

Single-cell transcriptome landscape of ovarian cells during primordial follicle assembly in mice.

Wang JJ, Ge W, Zhai QY, Liu JC, Sun XW, Liu WX, Li L, Lei CZ, Dyce PW, De Felici M, Shen W
PLoS biology 2020 Dec;18(12):e3001025

Intraflagellar transport protein 74 is essential for spermatogenesis and male fertility in mice†.

Shi L, Zhou T, Huang Q, Zhang S, Li W, Zhang L, Hess RA, Pazour GJ, Zhang Z
Biology of reproduction 2019 Jul 1;101(1):188-199

Intraflagellar transporter protein 140 (IFT140), a component of IFT-A complex, is essential for male fertility and spermiogenesis in mice.

Zhang Y, Liu H, Li W, Zhang Z, Zhang S, Teves ME, Stevens C, Foster JA, Campbell GE, Windle JJ, Hess RA, Pazour GJ, Zhang Z
Cytoskeleton (Hoboken, N.J.) 2018 Feb;75(2):70-84

IFT25, an intraflagellar transporter protein dispensable for ciliogenesis in somatic cells, is essential for sperm flagella formation.

Liu H, Li W, Zhang Y, Zhang Z, Shang X, Zhang L, Zhang S, Li Y, Somoza AV, Delpi B, Gerton GL, Foster JA, Hess RA, Pazour GJ, Zhang Z
Biology of reproduction 2017 May 1;96(5):993-1006

Upregulation and phosphorylation of HspB1/Hsp25 and HspB5/αB-crystallin after transient middle cerebral artery occlusion in rats.

Bartelt-Kirbach B, Slowik A, Beyer C, Golenhofen N
Cell stress & chaperones 2017 Jul;22(4):653-663

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Kanie T, Abbott KL, Mooney NA, Plowey ED, Demeter J, Jackson PK
Developmental cell 2017 Jul 10;42(1):22-36.e12

Intraflagellar transporter protein (IFT27), an IFT25 binding partner, is essential for male fertility and spermiogenesis in mice.

Zhang Y, Liu H, Li W, Zhang Z, Shang X, Zhang D, Li Y, Zhang S, Liu J, Hess RA, Pazour GJ, Zhang Z
Developmental biology 2017 Dec 1;432(1):125-139

Association between small heat shock protein B11 and the prognostic value of MGMT promoter methylation in patients with high-grade glioma.

Cheng W, Li M, Jiang Y, Zhang C, Cai J, Wang K, Wu A
Journal of neurosurgery 2016 Jul;125(1):7-16

Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.

Raman M, Sergeev M, Garnaas M, Lydeard JR, Huttlin EL, Goessling W, Shah JV, Harper JW
Nature cell biology 2015 Oct;17(10):1356-69

Intraflagellar transport 27 is essential for hedgehog signaling but dispensable for ciliogenesis during hair follicle morphogenesis.

Yang N, Li L, Eguether T, Sundberg JP, Pazour GJ, Chen J
Development (Cambridge, England) 2015 Jun 15;142(12):2194-202

IFT27 links the BBSome to IFT for maintenance of the ciliary signaling compartment.

Eguether T, San Agustin JT, Keady BT, Jonassen JA, Liang Y, Francis R, Tobita K, Johnson CA, Abdelhamed ZA, Lo CW, Pazour GJ
Developmental cell 2014 Nov 10;31(3):279-290

IFT25 links the signal-dependent movement of Hedgehog components to intraflagellar transport.

Keady BT, Samtani R, Tobita K, Tsuchya M, San Agustin JT, Follit JA, Jonassen JA, Subramanian R, Lo CW, Pazour GJ
Developmental cell 2012 May 15;22(5):940-51

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 15732-1-AP(HSPB11 antibody) at dilution of 1:500
Sample type: cell line