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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-35947 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Rat (98%), Zebrafish (82%), Xenopus laevis (92%), Pig (96%), Rabbit (98%), Chicken (93%), Rhesus Monkey (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.82 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGM1 Polyclonal Antibody detects PGM1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PGM1 Polyclonal Antibody (Product # PA5-35947) diluted by 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGM1 Polyclonal Antibody detects PGM1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PGM1 protein stained by PGM1 Polyclonal Antibody (Product # PA5-35947) diluted at 1:500. Blue: Hoechst 33342 staining.
