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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- H00001656-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001656-M01, RRID:AB_436960
- Product name
- DDX6 monoclonal antibody (M01), clone 3D2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant DDX6.
- Antigen sequence
MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGG
TQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGD
DWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKR
ELLMGIFEMGWEKPSPIQEESIPIALSGRDILARA
KNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRE
LALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIM
RLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVL
DEADKLLSQDFVQIMEDIILTLPKNRQILLYSATF
PLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAY
VTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELL
AKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLC
RNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLH
RIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGT
EIKPIPSNIDKSLYVAEYHSEPVEDEKP- Isotype
- IgG
- Antibody clone number
- 3D2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references DDX6 localizes to nuage structures and the annulus of mammalian spermatogenic cells.
The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome.
Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines.
Kawahara C, Yokota S, Fujita H
Histochemistry and cell biology 2014 Jan;141(1):111-21
Histochemistry and cell biology 2014 Jan;141(1):111-21
The DEAD-box RNA helicase DDX6 is required for efficient encapsidation of a retroviral genome.
Yu SF, Lujan P, Jackson DL, Emerman M, Linial ML
PLoS pathogens 2011 Oct;7(10):e1002303
PLoS pathogens 2011 Oct;7(10):e1002303
Rck/p54 interacts with APP mRNA as part of a multi-protein complex and enhances APP mRNA and protein expression in neuronal cell lines.
Broytman O, Westmark PR, Gurel Z, Malter JS
Neurobiology of aging 2009 Dec;30(12):1962-74
Neurobiology of aging 2009 Dec;30(12):1962-74
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DDX6 monoclonal antibody (M01), clone 3D2 Western Blot analysis of DDX6 expression in Hela ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2.Lane 1: DDX6 transfected lysate(53.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to DDX6 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
